Reference - Detail
| RRC ID | 47454 |
|---|---|
| Author | Honjo K, Mauthner SE, Wang Y, Skene JHP, Tracey WD Jr. |
| Title | Nociceptor-Enriched Genes Required for Normal Thermal Nociception. |
| Journal | Cell Rep |
| Abstract |
Here, we describe a targeted reverse genetic screen for thermal nociception genes in Drosophila larvae. Using laser capture microdissection and microarray analyses of nociceptive and non-nociceptive neurons, we identified 275 nociceptor-enriched genes. We then tested the function of the enriched genes with nociceptor-specific RNAi and thermal nociception assays. Tissue-specific RNAi targeted against 14 genes caused insensitive thermal nociception while targeting of 22 genes caused hypersensitive thermal nociception. Previously uncategorized genes were named for heat resistance (i.e., boilerman, fire dancer, oven mitt, trivet, thawb, and bunker gear) or heat sensitivity (firelighter, black match, eucalyptus, primacord, jet fuel, detonator, gasoline, smoke alarm, and jetboil). Insensitive nociception phenotypes were often associated with severely reduced branching of nociceptor neurites and hyperbranched dendrites were seen in two of the hypersensitive cases. Many genes that we identified are conserved in mammals. |
| Volume | 16(2) |
| Pages | 295-303 |
| Published | 2016-7-12 |
| DOI | 10.1016/j.celrep.2016.06.003 |
| PII | S2211-1247(16)30722-7 |
| PMID | 27346357 |
| PMC | PMC5333372 |
| MeSH | Animals Cells, Cultured Conserved Sequence Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / genetics* Evolution, Molecular Female Gene Knockdown Techniques Larva / cytology Larva / genetics Male Morphogenesis Nociception* Nociceptors / physiology* RNA Interference Taxis Response |
| IF | 8.109 |
| Times Cited | 24 |
| WOS Category | CELL BIOLOGY |
| Resource | |
| Drosophila | 10874R-3 11436R-1 11924R-3 12012R-3 12012R-1 12530R-2 14446R-2 14940R-2 14940R-1 15078R-2 15287R-1 15287R-2 1636R-3 1636R-2 1646R-3 17816R-2 2048R-1 2048R-3 2621R-3 2621R-1 30106R-3 3074R-1 32006R-3 32006R-1 3214R-1 10199R-1 3478R-1 3478R-2 4185R-2 4185R-3 4398R-1 4398R-3 4562R-1 4562R-2 4911R-1 6018R-1 6018R-3 6617R-3 6685R-3 6957R-1 6957R-3 7293R-2 7293R-1 9932R-2 |