RRC ID 47468
Author Zhao D, Liu XM, Yu ZQ, Sun LL, Xiong X, Dong MQ, Du LL.
Title Atg20- and Atg24-family proteins promote organelle autophagy in fission yeast.
Journal J Cell Sci
Abstract Autophagy cargos include not only soluble cytosolic materials but also bulky organelles, such as ER and mitochondria. In budding yeast, two proteins that contain the PX domain and the BAR domain, Atg20 and Atg24 (also known as Snx42 and Snx4, respectively) are required for organelle autophagy and contribute to general autophagy in a way that can be masked by compensatory mechanisms. It remains unclear why these proteins are important for organelle autophagy. Here, we show that in a distantly related fungal organism, the fission yeast Schizosaccharomyces pombe, autophagy of ER and mitochondria is induced by nitrogen starvation and is promoted by three Atg20- and Atg24-family proteins - Atg20, Atg24 and SPBC1711.11 (named here as Atg24b). These proteins localize at the pre-autophagosomal structure, or phagophore assembly site (PAS), during starvation. S. pombe Atg24 forms a homo-oligomer and acts redundantly with Atg20 and Atg24b, and the latter two proteins can form a hetero-oligomer. The organelle autophagy defect caused by the loss of these proteins is associated with a reduction of autophagosome size and a decrease in Atg8 accumulation at the PAS. These results provide new insights into the autophagic function of Atg20- and Atg24-family proteins.
Volume 129(22)
Pages 4289-4304
Published 2016-11-15
DOI 10.1242/jcs.194373
PII jcs.194373
PMID 27737912
MeSH Autophagosomes / metabolism Autophagosomes / ultrastructure Autophagy* Endoplasmic Reticulum / metabolism Mitochondria / metabolism Mutation / genetics Nitrogen / deficiency Organelles / metabolism* Protein Multimerization Schizosaccharomyces / cytology* Schizosaccharomyces / metabolism* Schizosaccharomyces / ultrastructure Schizosaccharomyces pombe Proteins / metabolism* Subcellular Fractions / metabolism
IF 4.517
Times Cited 15
WOS Category CELL BIOLOGY
Resource
DNA material pDUAL-CFH1c (RDB06148) pHIS3K-Ptub1 (RDB06564) S. pombe ORFeome clone ) SpYFH51F05 (SPW100525) SpYFH36C11 (SPW094459) SpYFH21B04 (SPW088428) SpYFH26D06 (SPW090478) SpYFH43H09 (SPW097377) SpYFH40C04 (SPW096052) SpYFH23C08 (SPW089256).