| RRC ID |
47476
|
| Author |
Kishimoto K, Nakayama M, Kinoshita M.
|
| Title |
In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes).
|
| Journal |
Dev Growth Differ
|
| Abstract |
The present study delineates the in vivo efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). VCre, SCre, and Cre RNA was microinjected into fertilized medaka eggs belonging to three transgenic lines harboring VloxP, SloxP, and loxP cassette. VCre induced site-specific recombination specifically at VloxP sequence and SCre at SloxP sequence without any cross-reactivity. These findings provide two novel alternative recombination systems in vivo in addition to the existing Cre/loxP and Flp/FRT systems, thus enabling sophisticated gene expression in model organisms.
|
| Volume |
58(6)
|
| Pages |
516-21
|
| Published |
2016-8-1
|
| DOI |
10.1111/dgd.12289
|
| PMID |
27224047
|
| MeSH |
Animals
Female
Gene Expression Regulation*
Genetic Engineering / methods*
Integrases*
Male
Oryzias* / genetics
Oryzias* / metabolism
Recombination, Genetic*
|
| IF |
1.723
|
| Times Cited |
2
|
|
WOS Category
|
CELL BIOLOGY
DEVELOPMENTAL BIOLOGY
|
| Resource |
| DNA material |
pactb-loxP-mCherry-loxP-EGFP (RDB14532)
pCS2-OlCre (RDB14533). |
