RRC ID 47495
Author Tanaka Y, Ono N, Shima T, Tanaka G, Katoh Y, Nakayama K, Takatsu H, Shin HW.
Title The phospholipid flippase ATP9A is required for the recycling pathway from the endosomes to the plasma membrane.
Journal Mol Biol Cell
Abstract Type IV P-type ATPases (P4-ATPases) are phospholipid flippases that translocate phospholipids from the exoplasmic (or luminal) to the cytoplasmic leaflet of lipid bilayers. In Saccharomyces cerevisiae, P4-ATPases are localized to specific subcellular compartments and play roles in compartment-mediated membrane trafficking; however, roles of mammalian P4-ATPases in membrane trafficking are poorly understood. We previously reported that ATP9A, one of 14 human P4-ATPases, is localized to endosomal compartments and the Golgi complex. In this study, we found that ATP9A is localized to phosphatidylserine (PS)-positive early and recycling endosomes, but not late endosomes, in HeLa cells. Depletion of ATP9A delayed the recycling of transferrin from endosomes to the plasma membrane, although it did not affect the morphology of endosomal structures. Moreover, depletion of ATP9A caused accumulation of glucose transporter 1 in endosomes, probably by inhibiting their recycling. By contrast, depletion of ATP9A affected neither the early/late endosomal transport and degradation of epidermal growth factor (EGF) nor the transport of Shiga toxin B fragment from early/recycling endosomes to the Golgi complex. Therefore ATP9A plays a crucial role in recycling from endosomes to the plasma membrane.
Volume 27(24)
Pages 3883-3893
Published 2016-12-1
DOI 10.1091/mbc.E16-08-0586
PII mbc.E16-08-0586
PMID 27733620
PMC PMC5170610
MeSH Adenosine Triphosphatases / metabolism Biological Transport Cell Membrane / metabolism Endosomes / metabolism* Endosomes / physiology Golgi Apparatus / metabolism HeLa Cells Humans Lipid Bilayers / metabolism Membrane Proteins / metabolism Phosphatidylserines / metabolism Phospholipid Transfer Proteins / metabolism* Phospholipid Transfer Proteins / physiology* Phospholipids / metabolism Protein Transport Transport Vesicles / metabolism
IF 3.905
Times Cited 21
DNA material pCMV-VSV-G-RSV-Rev (RDB04393)