RRC ID 47518
著者 Kawase M, He F, Kubota A, Miyazawa K, Yoda K, Hiramatsu M.
タイトル Strain-specific detection by pulsed-field gel electrophoresis of Lactobacillus gasseri TMC0356 in human feces after oral administration of these organisms.
ジャーナル Microbiol Immunol
Abstract The present study aimed to develop an innovative, strain-specific means of identifying the probiotic Lactobacillus gasseri TMC0356 and to determine whether orally administered TMC0356 could be recovered from the human intestine. High molecular weight genomic DNA was isolated from TMC0356 and 14 reference strains of L. gasseri, including the type strain. The DNA samples were digested with the selected rare-cutting restriction endonucleases SmaI, SacII and ApaI and the resulting fragments separated by pulsed-field gel electrophoresis (PFGE) in a size range between 20 to 290 kb. TMC0356 could be distinguished from the other L. gasseri strains on the basis of the SmaI and SacII macrorestriction patterns. Furthermore, L. gasseri strains isolated from the feces of subjects who had ingested TMC0356 were identical to TMC0356 in the SmaI, SacII and ApaI macrorestriction fragments of digested DNA. These results suggest that PFGE of genomic DNA digested with SmaI, SacII, could be a practical means of identification of TMC0356. Furthermore, these results indicate that ingested TMC0356 can survive in, and colonize, the human intestine.
巻・号 55(8)
ページ 589-94
公開日 2011-8-1
DOI 10.1111/j.1348-0421.2011.00350.x
PMID 21575044
MeSH Administration, Oral Adult Bacteriological Techniques / methods* DNA Restriction Enzymes / metabolism DNA, Bacterial / genetics DNA, Bacterial / metabolism Electrophoresis, Gel, Pulsed-Field / methods* Feces / microbiology* Humans Lactobacillus / classification Lactobacillus / genetics Lactobacillus / isolation & purification* Probiotics / administration & dosage*
IF 1.566
引用数 5
WOS 分野 MICROBIOLOGY IMMUNOLOGY
リソース情報
一般微生物 JCM 1025 JCM 1026 JCM 1031 JCM 1130 JCM 1131 JCM 5343 JCM 5344 JCM 5813 JCM 8787 JCM 8788 JCM 8789 JCM 8790 JCM 11046 JCM 11657