RRC ID |
47518
|
Author |
Kawase M, He F, Kubota A, Miyazawa K, Yoda K, Hiramatsu M.
|
Title |
Strain-specific detection by pulsed-field gel electrophoresis of Lactobacillus gasseri TMC0356 in human feces after oral administration of these organisms.
|
Journal |
Microbiol Immunol
|
Abstract |
The present study aimed to develop an innovative, strain-specific means of identifying the probiotic Lactobacillus gasseri TMC0356 and to determine whether orally administered TMC0356 could be recovered from the human intestine. High molecular weight genomic DNA was isolated from TMC0356 and 14 reference strains of L. gasseri, including the type strain. The DNA samples were digested with the selected rare-cutting restriction endonucleases SmaI, SacII and ApaI and the resulting fragments separated by pulsed-field gel electrophoresis (PFGE) in a size range between 20 to 290 kb. TMC0356 could be distinguished from the other L. gasseri strains on the basis of the SmaI and SacII macrorestriction patterns. Furthermore, L. gasseri strains isolated from the feces of subjects who had ingested TMC0356 were identical to TMC0356 in the SmaI, SacII and ApaI macrorestriction fragments of digested DNA. These results suggest that PFGE of genomic DNA digested with SmaI, SacII, could be a practical means of identification of TMC0356. Furthermore, these results indicate that ingested TMC0356 can survive in, and colonize, the human intestine.
|
Volume |
55(8)
|
Pages |
589-94
|
Published |
2011-8-1
|
DOI |
10.1111/j.1348-0421.2011.00350.x
|
PMID |
21575044
|
MeSH |
Administration, Oral
Adult
Bacteriological Techniques / methods*
DNA Restriction Enzymes / metabolism
DNA, Bacterial / genetics
DNA, Bacterial / metabolism
Electrophoresis, Gel, Pulsed-Field / methods*
Feces / microbiology*
Humans
Lactobacillus / classification
Lactobacillus / genetics
Lactobacillus / isolation & purification*
Probiotics / administration & dosage*
|
IF |
1.566
|
Times Cited |
5
|
WOS Category
|
MICROBIOLOGY
IMMUNOLOGY
|
Resource |
General Microbes |
JCM 1025
JCM 1026
JCM 1031
JCM 1130
JCM 1131
JCM 5343
JCM 5344
JCM 5813
JCM 8787
JCM 8788
JCM 8789
JCM 8790
JCM 11046
JCM 11657 |