| RRC ID |
4755
|
| Author |
Rosshart S, Hofmann M, Schweier O, Pfaff AK, Yoshimoto K, Takeuchi T, Molnar E, Schamel WW, Pircher H.
|
| Title |
Interaction of KLRG1 with E-cadherin: new functional and structural insights.
|
| Journal |
Eur J Immunol
|
| Abstract |
The killer cell lectin-like receptor G1 (KLRG1) is an inhibitory receptor expressed by memory T cells and NK cells in man and mice. It is frequently used as a cell differentiation marker and members of the cadherin family are ligands for KLRG1. The present study provides new insights into the interaction of mouse KLRG1 with E-cadherin. Firstly, we demonstrate that co-engagement of KLRG1 and CD3/TCR in a spatially linked manner was required for inhibition arguing against the notion that KLRG1-ligation per se transmits inhibitory signals. Secondly, experiments with T cells carrying Y(7)F-mutant KLRG1 molecules with a replacement of the tyrosine residue to phenylalanine in the single ITIM indicated that the inhibitory activity of KLRG1 is counteracted to some degree by increased interaction of KLRG1(+) T cells with E-cadherin expressing target cells. Thirdly, we demonstrate that deletion of the first or the second external domain of E-cadherin abolished reactivity in KLRG1-reporter cell assays. Finally, we made the intriguing observation that KLRG1 formed multimeric protein complexes in T cells in addition to the previously described mono- and dimeric molecules.
|
| Volume |
38(12)
|
| Pages |
3354-64
|
| Published |
2008-12-1
|
| DOI |
10.1002/eji.200838690
|
| PMID |
19009530
|
| MeSH |
Animals
Antibodies / immunology
Antigen-Presenting Cells / immunology
CD3 Complex / immunology
Cadherins / genetics
Cadherins / metabolism*
Cell Line
Gene Deletion
Genes, Reporter / genetics
Lectins, C-Type
Mice
Mutation / genetics
Protein Binding
Protein Multimerization
Receptors, Antigen, T-Cell / immunology
Receptors, Immunologic / genetics
Receptors, Immunologic / metabolism*
|
| IF |
4.404
|
| Times Cited |
41
|
|
WOS Category
|
IMMUNOLOGY
|
| Resource |
| DNA material |
Mouse E-cadherin full length cDNA (RDB01184) |
