| RRC ID |
4758
|
| 著者 |
Nagaoka M, Hagiwara Y, Takemura K, Murakami Y, Li J, Duncan SA, Akaike T.
|
| タイトル |
Design of the artificial acellular feeder layer for the efficient propagation of mouse embryonic stem cells.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Embryonic stem (ES) cells are pluripotent-undifferentiated cells that have a great interest for the investigation of developmental biology. Murine ES cells maintain their pluripotency by the supplementation of the leukemia inhibitory factor (LIF). LIF is reported to act as a matrix-anchored form, and immobilized cytokines are useful to sustain their signaling on target cells. In this study, we used the immobilizable fusion protein composed of LIF and IgG-Fc region, which was used as a model of the matrix-anchored form of LIF to establish a novel system for ES cell culture and to investigate the effect of immobilized LIF on maintenance of ES cell pluripotency. Mouse ES cells maintained their undifferentiated state on the surface coated with LIF-Fc. Furthermore, when cultured on the co-immobilized surface with LIF-Fc and E-cadherin-Fc, mouse ES cells showed characteristic scattering morphologies without colony formation, and they could maintain their undifferentiated state and pluripotency without additional LIF supplementation. The activation of LIF signaling was sustained on the co-immobilized surface. These results indicate that immobilized LIF and E-cadherin can maintain mouse ES cells efficiently and that the immobilizable LIF-Fc fusion protein is useful for the investigation of signaling pathways of an immobilized form of LIF in the maintenance of ES cell pluripotency.
|
| 巻・号 |
283(39)
|
| ページ |
26468-76
|
| 公開日 |
2008-9-26
|
| DOI |
10.1074/jbc.M805037200
|
| PII |
S0021-9258(20)52390-9
|
| PMID |
18614540
|
| PMC |
PMC3258920
|
| MeSH |
Animals
Cadherins / chemistry
Cadherins / genetics
Cadherins / metabolism
Cell Culture Techniques / methods*
Cell Line
Embryonic Stem Cells / cytology*
Extracellular Matrix / chemistry
Extracellular Matrix / genetics
Extracellular Matrix / metabolism
Immunoglobulin Constant Regions / chemistry
Immunoglobulin Constant Regions / genetics
Immunoglobulin Constant Regions / pharmacology*
Leukemia Inhibitory Factor / chemistry
Leukemia Inhibitory Factor / genetics
Leukemia Inhibitory Factor / pharmacology*
Mice
Pluripotent Stem Cells / cytology*
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / pharmacology
Signal Transduction / drug effects
|
| IF |
4.238
|
| 引用数 |
26
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
Mouse E-cadherin full length cDNA (RDB01184) |
