RRC ID 4762
Author Nagaoka M, Koshimizu U, Yuasa S, Hattori F, Chen H, Tanaka T, Okabe M, Fukuda K, Akaike T.
Title E-cadherin-coated plates maintain pluripotent ES cells without colony formation.
Journal PLoS ONE
Abstract Embryonic stem (ES) cells cultured on gelatin-coated plates or feeder layers form tight aggregated colonies by the E-cadherin-mediated cell-cell adhesions. Here we show that murine ES cells do not make cell-cell contacts or form colonies when cultured on the plate coated with a fusion protein of E-cadherin and IgG Fc domain. The cells in culture retain all ES cell features including pluripotency to differentiate into cells of all three germ layers and germ-line transmission after extended culture. Furthermore, they show a higher proliferative ability, lower dependency on LIF, and higher transfection efficiency than colony-forming conditions. Our results suggest that aggregated colony formation might inhibit diffusion of soluble factors and increase cell-cell communication, which may result in a heterogeneous environment within and between surrounding cells of the colony. This method should enable more efficient and scalable culture of ES cells, an important step towards the clinical application of these cells.
Volume 1
Pages e15
Published 2006-12-20
DOI 10.1371/journal.pone.0000015
PMID 17183641
PMC PMC1762325
MeSH Animals Cadherins / physiology* Cell Adhesion / physiology Cell Aggregation / physiology Cell Culture Techniques / methods Cell Differentiation Cell Proliferation Cells, Cultured Coated Materials, Biocompatible Colony-Forming Units Assay Embryonic Stem Cells / cytology* Embryonic Stem Cells / physiology* Embryonic Stem Cells / transplantation Male Mice Mice, Nude Phenotype Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / physiology* Pluripotent Stem Cells / transplantation Recombinant Fusion Proteins / metabolism Signal Transduction Teratoma / etiology Testicular Neoplasms / etiology Transfection
IF 2.766
Times Cited 69
WOS Category CELL BIOLOGY
Resource
DNA material Mouse E-cadherin full length cDNA (RDB01184)