RRC ID 47632
Author Yokoyama C, Yajima C, Machida T, Kawahito Y, Uchida M, Hisatomi H.
Title Interleukin-8 enhances the effect of colchicine on cell death.
Journal Biochem. Biophys. Res. Commun.
Abstract Pro-inflammatory cytokines are known to be generated in tumors and play important roles in angiogenesis, mitosis, and tumor progression. However, few studies have investigated the synergistic effects of pro-inflammatory cytokines and anticancer drugs on cell death. In the present study, we examined the combined effects of pro-inflammatory cytokines and colchicine on cell death of cancer cells. Colchicine induces G2/M arrest in the cell cycle by binding to tubulin, one of the main constituents of microtubules. SUIT-2 human pancreatic cancer cell line cells overexpressing pro-inflammatory cytokines, including interleukin (IL)-1β, IL-8, and tumor necrosis factor (TNF)-α, were treated with colchicine. The effect of colchicine on cell death was enhanced in cells overexpressing IL-8. Moreover, the effect of colchicine on cell death was enhanced in cells overexpressing two IL-8 up-regulators, NF-κB and IL-6, but not in cells overexpressing an IL-8 down-regulator, splicing factor proline/glutamine-rich (SFPQ). Synergistic effects of IL-8 and colchicine were also observed in cells overexpressing IL-8 isoforms lacking the signal peptide. Therefore, IL-8 appeared to function as an enhancer of cell death in cancer cells treated with colchicine. The present results suggest a new role for IL-8 related to cell death of cancer cells.
Volume 485(1)
Pages 89-94
Published 2017-3-25
DOI 10.1016/j.bbrc.2017.02.025
PII S0006-291X(17)30286-3
PMID 28189686
MeSH Antineoplastic Agents / pharmacology* Cell Death / drug effects* Cell Line, Tumor Colchicine / pharmacology* Humans Interleukin-8 / genetics Interleukin-8 / immunology* Pancreas / drug effects Pancreas / immunology Pancreas / metabolism Pancreas / pathology Pancreatic Neoplasms / drug therapy* Pancreatic Neoplasms / genetics Pancreatic Neoplasms / immunology* Pancreatic Neoplasms / pathology Up-Regulation
IF 2.559
Times Cited 1
DNA material pCMFlag_hsIL8 (RDB07023) pCMFlag_hsIL6 (RDB06998) pCMFlag_hsIL1B (RDB06666) pBluescript SKII+hTNF alpha (RDB01477).