論文 - 詳細
| RRC ID | 47679 |
|---|---|
| 著者 | Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J. |
| タイトル | Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. |
| ジャーナル | PLoS One |
| Abstract |
Imaging the activities of individual neurons with genetically encoded Ca(2+) indicators (GECIs) is a promising method for understanding neuronal network functions. Here, we report GECIs with improved neuronal Ca(2+) signal detectability, termed G-CaMP6 and G-CaMP8. Compared to a series of existing G-CaMPs, G-CaMP6 showed fairly high sensitivity and rapid kinetics, both of which are suitable properties for detecting subtle and fast neuronal activities. G-CaMP8 showed a greater signal (F(max)/F(min) = 38) than G-CaMP6 and demonstrated kinetics similar to those of G-CaMP6. Both GECIs could detect individual spikes from pyramidal neurons of cultured hippocampal slices or acute cortical slices with 100% detection rates, demonstrating their superior performance to existing GECIs. Because G-CaMP6 showed a higher sensitivity and brighter baseline fluorescence than G-CaMP8 in a cellular environment, we applied G-CaMP6 for Ca(2+) imaging of dendritic spines, the putative postsynaptic sites. By expressing a G-CaMP6-actin fusion protein for the spines in hippocampal CA3 pyramidal neurons and electrically stimulating the granule cells of the dentate gyrus, which innervate CA3 pyramidal neurons, we found that sub-threshold stimulation triggered small Ca(2+) responses in a limited number of spines with a low response rate in active spines, whereas supra-threshold stimulation triggered large fluorescence responses in virtually all of the spines with a 100% activity rate. |
| 巻・号 | 7(12) |
| ページ | e51286 |
| 公開日 | 2012-1-1 |
| DOI | 10.1371/journal.pone.0051286 |
| PII | PONE-D-12-27822 |
| PMID | 23240011 |
| PMC | PMC3519846 |
| MeSH | Animals Calcium* / chemistry Calcium* / isolation & purification Calcium* / metabolism Calcium Signaling / genetics* Cells, Cultured Dendritic Spines / metabolism Dentate Gyrus / cytology Dentate Gyrus / metabolism Green Fluorescent Proteins* / chemistry Green Fluorescent Proteins* / genetics HeLa Cells Humans Indicators and Reagents / analysis Indicators and Reagents / metabolism Neuroanatomical Tract-Tracing Techniques Neurons / cytology Neurons / metabolism Pyramidal Cells* / cytology Pyramidal Cells* / metabolism Rats |
| IF | 2.74 |
| 引用数 | 104 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | G-CaMP6 (RDB14609) G-CaMP7 (RDB14610) G-CaMP8 (RDB14611). |