RRC ID 47701
Author Liu L, Kamei KI, Yoshioka M, Nakajima M, Li J, Fujimoto N, Terada S, Tokunaga Y, Koyama Y, Sato H, Hasegawa K, Nakatsuji N, Chen Y.
Title Nano-on-micro fibrous extracellular matrices for scalable expansion of human ES/iPS cells.
Journal Biomaterials
Abstract Human pluripotent stem cells (hPSCs) hold great potential for industrial and clinical applications. Clinical-grade scaffolds and high-quality hPSCs are required for cell expansion as well as easy handling and manipulation of the products. Current hPSC culture methods do not fulfill these requirements because of a lack of proper extracellular matrices (ECMs) and cell culture wares. We developed a layered nano-on-micro fibrous cellular matrix mimicking ECM, named "fiber-on-fiber (FF)" matrix, which enables easy handling and manipulation of cultured cells. While non-woven sheets of cellulose and polyglycolic acid were used as a microfiber layer facilitating mechanical stability, electrospun gelatin nanofibers were crosslinked on the microfiber layer, generating a mesh structure with connected nanofibers facilitating cell adhesion and growth. Our results showed that the FF matrix supports effective hPSC culture with maintenance of their pluripotency and normal chromosomes over two months, as well as effective scaled-up expansion, with fold increases of 54.1 ± 15.6 and 40.4 ± 8.4 in cell number per week for H1 human embryonic stem cells and 253G1 human induced pluripotent stem cells, respectively. This simple approach to mimick the ECM may have important implications after further optimization to generate lineage-specific products.
Volume 124
Pages 47-54
Published 2017-4
DOI 10.1016/j.biomaterials.2017.01.039
PII S0142-9612(17)30062-5
PMID 28187394
MeSH Batch Cell Culture Techniques / methods* Biomimetic Materials / chemistry Cell Differentiation / physiology Cell Proliferation / physiology Cells, Cultured Extracellular Matrix / chemistry* Human Embryonic Stem Cells / cytology Human Embryonic Stem Cells / physiology* Humans Nanofibers / chemistry* Nanofibers / ultrastructure Pluripotent Stem Cells / cytology Pluripotent Stem Cells / physiology* Tissue Engineering / instrumentation Tissue Engineering / methods* Tissue Scaffolds*
IF 10.273
Times Cited 14
Human and Animal Cells 253G1(HPS0002)