RRC ID 47726
著者 Kojo K, Yaeno T, Kusumi K, Matsumura H, Fujisawa S, Terauchi R, Iba K.
タイトル Regulatory mechanisms of ROI generation are affected by rice spl mutations.
ジャーナル Plant Cell Physiol
Abstract Reactive oxygen intermediates (ROIs) play a pivotal role in the hypersensitive response (HR) in disease resistance. NADPH oxidase is a major source of ROI; however, the mechanisms of its regulation are unclear. Rice spl mutants spontaneously form lesions which resemble those occurring during the HR, suggesting that the mutations affect regulation of the HR. We found that spl2, spl7 and spl11 mutant cells accumulated increased amounts of H(2)O(2) in response to rice blast fungal elicitor. Increased accumulation of ROIs was suppressed by inhibition of NADPH oxidase in the spl cells, and was also observed in the ozone-exposed spl plants. These mutants have sufficient activities of ROI-scavenging enzymes compared with the wild type. In addition, spl7 mutant cells accumulated higher amounts of H(2)O(2) when treated with calyculin A (CA), an inhibitor of protein phosphatase. Furthermore, spl2 mutant plants exhibited accelerated accumulation of H(2)O(2) and increased rates of cell death in response to wounding. These results suggest that the spl2, spl7 and spl11 mutants are defective in the regulation of NADPH oxidase, and the spl7 mutation may give rise to enhancement of the signaling pathway which protein dephosphorylation controls, while the spl2 mutation affects both the pathogen-induced and wound-induced signaling pathways.
巻・号 47(8)
ページ 1035-44
公開日 2006-8-1
DOI 10.1093/pcp/pcj074
PII pcj074
PMID 16816407
MeSH Cell Death / physiology Cells, Cultured Enzyme Inhibitors / pharmacology Hydrogen Peroxide / metabolism* Hypersensitivity Marine Toxins Mutation Oryza / cytology* Oxazoles / pharmacology Ozone / pharmacology Phosphoprotein Phosphatases / antagonists & inhibitors Reactive Oxygen Species / metabolism* Signal Transduction / physiology* Wounds and Injuries / pathology
IF 4.062
引用数 22
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
イネ 標識遺伝子系統