RRC ID 47755
Author Hayashi S, Kawaguchi A, Uchiyama I, Kawasumi-Kita A, Kobayashi T, Nishide H, Tsutsumi R, Tsuru K, Inoue T, Ogino H, Agata K, Tamura K, Yokoyama H.
Title Epigenetic modification maintains intrinsic limb-cell identity in Xenopus limb bud regeneration.
Journal Dev Biol
Abstract Many amphibians can regenerate limbs, even in adulthood. If a limb is amputated, the stump generates a blastema that makes a complete, new limb in a process similar to developmental morphogenesis. The blastema is thought to inherit its limb-patterning properties from cells in the stump, and it retains the information despite changes in morphology, gene expression, and differentiation states required by limb regeneration. We hypothesized that these cellular properties are maintained as epigenetic memory through histone modifications. To test this hypothesis, we analyzed genome-wide histone modifications in Xenopus limb bud regeneration. The trimethylation of histone H3 at lysine 4 (H3K4me3) is closely related to an open chromatin structure that allows transcription factors access to genes, whereas the trimethylation of histone H3 at lysine 27 (H3K27me3) is related to a closed chromatin state that blocks the access of transcription factors. We compared these two modification profiles by high-throughput sequencing of samples prepared from the intact limb bud and the regenerative blastema by chromatin immunoprecipitation. For many developmental genes, histone modifications at the transcription start site were the same in the limb bud and the blastema, were stable during regeneration, and corresponded well to limb properties. These results support our hypothesis that histone modifications function as a heritable cellular memory to maintain limb cell properties, despite dynamic changes in gene expression during limb bud regeneration in Xenopus.
Volume 406(2)
Pages 271-82
Published 2015-10-15
DOI 10.1016/j.ydbio.2015.08.013
PII S0012-1606(15)30130-5
PMID 26282893
MeSH Adenosine / analogs & derivatives Animals Animals, Genetically Modified Base Sequence Chromatin Immunoprecipitation DNA Methylation / genetics DNA Primers / genetics Epigenesis, Genetic / physiology* Fluorescent Antibody Technique Gene Expression Regulation, Developmental / physiology* High-Throughput Nucleotide Sequencing Histone Code / physiology* Histones / metabolism In Situ Hybridization Limb Buds / physiology* Molecular Sequence Data Polymerase Chain Reaction Regeneration / physiology* Sequence Analysis, DNA Xenopus / physiology*
IF 2.896
Times Cited 17
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Clawed frogs / Newts X. tropicalis