RRC ID 47760
Author Müller F, Xu J, Kristensen L, Wolters-Arts M, de Groot PF, Jansma SY, Mariani C, Park S, Rieu I.
Title High-Temperature-Induced Defects in Tomato (Solanum lycopersicum) Anther and Pollen Development Are Associated with Reduced Expression of B-Class Floral Patterning Genes.
Journal PLoS ONE
Abstract Sexual reproduction is a critical process in the life-cycle of plants and very sensitive to environmental perturbations. To better understand the effect of high temperature on plant reproduction, we cultivated tomato (Solanum lycopersicum) plants in continuous mild heat. Under this condition we observed a simultaneous reduction in pollen viability and appearance of anthers with pistil-like structures, while in a more thermotolerant genotype, both traits were improved. Ectopic expression of two pistil-specific genes, TRANSMITTING TISSUE SPECIFIC and TOMATO AGAMOUS LIKE11, in the anthers confirmed that the anthers had gained partial pistil identity. Concomitantly, expression of the B-class genes TOMATO APETALA3, TOMATO MADS BOX GENE6 (TM6) and LePISTILLATA was reduced in anthers under continuous mild heat. Plants in which TM6 was partially silenced reacted hypersensitively to temperature elevation with regard to the frequency of pistilloid anthers, pollen viability and pollen quantity. Taken together, these results suggest that high-temperature-induced down-regulation of tomato B-class genes contributes to anther deformations and reduced male fertility. Improving our understanding of how temperature perturbs the molecular mechanisms of anther and pollen development will be important in the view of maintaining agricultural output under current climate changes.
Volume 11(12)
Pages e0167614
Published 2016
DOI 10.1371/journal.pone.0167614
PII PONE-D-16-06227
PMID 27936079
PMC PMC5147909
MeSH Cell Survival Climate Change Down-Regulation Flowers / genetics Flowers / growth & development Flowers / ultrastructure Gene Expression Regulation, Plant* Genes, Plant Hot Temperature Lycopersicon esculentum / genetics Lycopersicon esculentum / growth & development* Lycopersicon esculentum / ultrastructure Plant Proteins / genetics Pollen / cytology Pollen / genetics Pollen / growth & development*
IF 2.776
Times Cited 2
WOS Category PLANT SCIENCES
Resource
Tomato TOMJPF00001