Reference - Detail
| RRC ID | 4776 |
|---|---|
| Author | Imai Y, Soda M, Inoue H, Hattori N, Mizuno Y, Takahashi R. |
| Title | An unfolded putative transmembrane polypeptide, which can lead to endoplasmic reticulum stress, is a substrate of Parkin. |
| Journal | Cell |
| Abstract |
A putative G protein-coupled transmembrane polypeptide, named Pael receptor, was identified as an interacting protein with Parkin, a gene product responsible for autosomal recessive juvenile Parkinsonism (AR-JP). When overexpressed in cells, this receptor tends to become unfolded, insoluble, and ubiquitinated in vivo. The insoluble Pael receptor leads to unfolded protein-induced cell death. Parkin specifically ubiquitinates this receptor in the presence of ubiquitin-conjugating enzymes resident in the endoplasmic reticulum and promotes the degradation of insoluble Pael receptor, resulting in suppression of the cell death induced by Pael receptor overexpression. Moreover, the insoluble form of Pael receptor accumulates in the brains of AR-JP patients. Here, we show that the unfolded Pael receptor is a substrate of Parkin, the accumulation of which may cause selective neuronal death in AR-JP. |
| Volume | 105(7) |
| Pages | 891-902 |
| Published | 2001-6-29 |
| DOI | 10.1016/s0092-8674(01)00407-x |
| PII | S0092-8674(01)00407-X |
| PMID | 11439185 |
| MeSH | Acetylcysteine / analogs & derivatives* Acetylcysteine / pharmacology Brain / cytology Brain / metabolism Cell Death Cells, Cultured Cysteine Proteinase Inhibitors / pharmacology Endoplasmic Reticulum / metabolism* Humans Ligases / genetics Ligases / metabolism* Microscopy, Fluorescence Parkinsonian Disorders / genetics Parkinsonian Disorders / metabolism* Precipitin Tests Protein Binding Protein Folding* Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism* Recombinant Fusion Proteins / metabolism Two-Hybrid System Techniques Ubiquitin-Conjugating Enzymes* Ubiquitin-Protein Ligases* Ubiquitins / metabolism |
| IF | 38.637 |
| Times Cited | 802 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| DNA material | Human ETA receptor cDNA (RDB01302) Human ETB receptor cDNA (RDB01303) pcDNA3-hPaelR (RDB02865). |