RRC ID 4777
Author Takatsu H, Tasaki H, Kim HN, Ueda S, Tsutsui M, Yamashita K, Toyokawa T, Morimoto Y, Nakashima Y, Adachi T.
Title Overexpression of EC-SOD suppresses endothelial-cell-mediated LDL oxidation.
Journal Biochem Biophys Res Commun
Abstract Reactive oxygen species have been proposed to play important roles in atherosclerosis. To investigate the protective role of extracellular superoxide dismutase (EC-SOD), its inhibition of endothelial-cell-mediated LDL oxidation was examined. We constructed the recombinant adenovirus AxCAEC-SOD expressing human EC-SOD by CAG promoter. Infection of endothelial cells with AxCAEC-SOD resulted in EC-SOD protein secretion in a dose-dependent manner and a decrease of endothelial-cell-derived superoxide production. Moreover, it was proven to coexist with heparan sulfate by immunohistochemical staining. Endothelial-cell-mediated LDL oxidation enhanced by ferric-sodium EDTA was inhibited by 47% in TBARS formation by AxCAEC-SOD infection. In agarose gel electrophoresis, AxCAEC-SOD decreased the negative charge of oxidized LDL by 50% and suppressed fragmentation of apolipoprotein B. These results suggested that human EC-SOD localized in the extracellular space and reduced endothelial-cell-mediated LDL oxidation. In subendothelial space, EC-SOD bound on heparan sulfate might suppress LDL oxidation through reduction of superoxide anion.
Volume 285(1)
Pages 84-91
Published 2001-7-6
DOI 10.1006/bbrc.2001.5114
PII S0006-291X(01)95114-9
PMID 11437376
MeSH Animals Base Sequence DNA Primers Electrophoresis, Polyacrylamide Gel Endothelium, Vascular / enzymology* Endothelium, Vascular / physiology* Heparitin Sulfate / metabolism Immunohistochemistry Kinetics Lipoproteins, LDL / metabolism* Oxidation-Reduction Recombinant Proteins / metabolism Superoxide Dismutase / metabolism* Swine
IF 2.985
Times Cited 44
DNA material pAxCAwt (RDB1678)