Reference - Detail
| RRC ID | 47770 |
|---|---|
| Author | Ueta R, Abe C, Watanabe T, Sugano SS, Ishihara R, Ezura H, Osakabe Y, Osakabe K. |
| Title | Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9. |
| Journal | Sci Rep |
| Abstract |
Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into SlIAA9-a key gene controlling parthenocarpy-with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome. Regenerated mutants exhibited morphological changes in leaf shape and seedless fruit-a characteristic of parthenocarpic tomato. And the segregated next generation (T1) also showed a severe phenotype associated with the homozygous mutated genome. The system developed here could be applied to produce parthenocarpic tomato in a wide variety of cultivars, as well as other major horticultural crops, using this precise and rapid breeding technique. |
| Volume | 7(1) |
| Pages | 507 |
| Published | 2017-3-30 |
| DOI | 10.1038/s41598-017-00501-4 |
| PII | 10.1038/s41598-017-00501-4 |
| PMID | 28360425 |
| PMC | PMC5428692 |
| MeSH | Base Sequence Breeding* CRISPR-Cas Systems / genetics* Gene Knockout Techniques Mutagenesis, Site-Directed Mutation / genetics Parthenogenesis / genetics* Phenotype Plants, Genetically Modified RNA, Guide, Kinetoplastida / genetics Solanum lycopersicum / genetics* Solanum lycopersicum / growth & development* |
| IF | 3.998 |
| Times Cited | 67 |
| WOS Category | PLANT SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 82 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Tomato | TOMJPF00001 |