Reference - Detail
| RRC ID | 4793 |
|---|---|
| Author | Ariizumi T, Takahashi S, Chan TC, Ito Y, Michiue T, Asashima M. |
| Title | Isolation and differentiation of Xenopus animal cap cells. |
| Journal | Curr Protoc Stem Cell Biol |
| Abstract |
Xenopus is used as a model animal for investigating the inductive events and organogenesis that occur during early vertebrate development. Given that they are easy to obtain in high numbers and are relatively large in size, Xenopus embryos are excellent specimens for performing manipulations such as microinjection and microsurgery. The animal cap, which is the area around the animal pole of the blastula, is destined to form the ectoderm during normal development. However, these cells retain pluripotentiality and upon exposure to specific inducers, the animal cap can differentiate into neural, mesodermal, and endodermal tissues. In this sense, the cells of the animal cap are equivalent to mammalian embryonic stem cells. In this unit, the isolation and differentiation of animal cap cells, the so-called animal cap assay, is described. Useful methods for analyzing the mechanism of animal cap differentiation at the molecular level are also described. |
| Volume | Chapter 1 |
| Pages | Unit 1D.5 |
| Published | 2009-4-1 |
| DOI | 10.1002/9780470151808.sc01d05s9 |
| PMID | 19382122 |
| MeSH | Animals Blastula / cytology* Cell Differentiation* Cell Separation* Cytological Techniques Embryo, Nonmammalian Models, Animal Pluripotent Stem Cells / cytology Xenopus |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
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| Clawed frogs / Newts | |