RRC ID |
4795
|
著者 |
Oka T, Miyahara M, Yamamoto J, Mitsui N, Fujii T, Tooi O, Kashiwagi K, Takase M, Kashiwagi A, Iguchi T.
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タイトル |
Application of metamorphosis assay to a native Japanese amphibian species, Rana rugosa, for assessing effects of thyroid system affecting chemicals.
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ジャーナル |
Ecotoxicol Environ Saf
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Abstract |
The aims of this study were to assess the utility of a metamorphosis assay for detecting thyroid hormone-disrupting chemicals using Rana rugosa, a domestic frog species in Japan, and to compare species differences in sensitivity to thyroxine (T(4)) and propylthiouracil (PTU) among R. rugosa, Xenopus laevis and Xenopus (Silurana) tropicalis. Tadpoles of R. rugosa (TK stages III/IV) were exposed to standard test chemicals for acceleration (T(4)) and inhibition (PTU) of metamorphosis for 28 days in semi-static condition and total body length and developmental stage (TK stage) were recorded every week. T(4) (0.61 and 2.24 microg/L in actual concentrations) and PTU (19.73, 76.83, and 155.67 mg/L in actual concentrations) induced significant acceleration and inhibition of metamorphosis, respectively. The present results indicate that the metamorphosis assay is successfully applied to the domestic frog species, R. rugosa, suggesting this assay can be used for the assessment of chemicals on ecological impacts in wild frog species.
|
巻・号 |
72(5)
|
ページ |
1400-5
|
公開日 |
2009-7-1
|
DOI |
10.1016/j.ecoenv.2009.03.012
|
PII |
S0147-6513(09)00054-2
|
PMID |
19394694
|
MeSH |
Animals
Antithyroid Agents / toxicity*
Biological Assay*
Dose-Response Relationship, Drug
Endocrine Disruptors / toxicity*
Feasibility Studies
Female
Larva / drug effects
Male
Metamorphosis, Biological / drug effects*
Propylthiouracil / toxicity
Ranidae / embryology
Ranidae / growth & development*
Species Specificity
Temperature
Thyroid Gland / drug effects*
Thyroxine / toxicity
Time Factors
Tissue Culture Techniques
Toxicity Tests / methods*
Xenopus laevis / growth & development
|
IF |
4.872
|
引用数 |
16
|
WOS 分野
|
TOXICOLOGY
ENVIRONMENTAL SCIENCES
|
リソース情報 |
ツメガエル・イモリ |
|