RRC ID 47965
Author Yu T, Graf M, Renn J, Schartl M, Larionova D, Huysseune A, Witten PE, Winkler C.
Title A vertebrate-specific and essential role for osterix in osteogenesis revealed by gene knockout in the teleost medaka.
Journal Development
Abstract osterix (osx; sp7) encodes a zinc-finger transcription factor that controls osteoblast differentiation in mammals. Although identified in all vertebrate lineages, its role in non-mammalian bone formation remains elusive. Here, we show that an osx mutation in medaka results in severe bone defects and larval lethality. Pre-osteoblasts fail to differentiate leading to severe intramembranous and perichondral ossification defects. The notochord sheath mineralizes normally, supporting the idea of an osteoblast-independent mechanism for teleost vertebral centra formation. This study establishes a key role for Osx for bone formation in a non-mammalian species, and reveals conserved and non-conserved features in vertebrate bone formation.
Volume 144(2)
Pages 265-271
Published 2017-1-15
DOI 10.1242/dev.139550
PII dev.139550
PMID 27993982
MeSH Animals Animals, Genetically Modified Calcification, Physiologic / genetics Gene Expression Regulation, Developmental Gene Knockout Techniques Notochord / embryology Oryzias / embryology* Oryzias / genetics* Osteogenesis / genetics* Phylogeny Sp7 Transcription Factor Species Specificity Transcription Factors / genetics Transcription Factors / physiology* Vertebrates / embryology Vertebrates / genetics Zebrafish Proteins / physiology
IF 5.611
Times Cited 17
Medaka osx_tv1mutant/osx:mCherry (TG1138) col10a1:nlGFP (TG1129)