RRC ID 47975
Author Matsuda R, Iehisa JCM, Sakaguchi K, Ohno R, Yoshida K, Takumi S.
Title Global gene expression profiling related to temperature-sensitive growth abnormalities in interspecific crosses between tetraploid wheat and Aegilops tauschii.
Journal PLoS One
Abstract Triploid wheat hybrids between tetraploid wheat and Aegilops tauschii sometimes show abnormal growth phenotypes, and the growth abnormalities inhibit generation of wheat synthetic hexaploids. In type II necrosis, one of the growth abnormalities, necrotic cell death accompanied by marked growth repression occurs only under low temperature conditions. At normal temperature, the type II necrosis lines show grass-clump dwarfism with no necrotic symptoms, excess tillers, severe dwarfism and delayed flowering. Here, we report comparative expression analyses to elucidate the molecular mechanisms of the temperature-dependent phenotypic plasticity in the triploid wheat hybrids. We compared gene and small RNA expression profiles in crown tissues to characterize the temperature-dependent phenotypic plasticity. No up-regulation of defense-related genes was observed under the normal temperature, and down-regulation of wheat APETALA1-like MADS-box genes, considered to act as flowering promoters, was found in the grass-clump dwarf lines. Some microRNAs, including miR156, were up-regulated, whereas the levels of transcripts of the miR156 target genes SPLs, known to inhibit tiller and branch number, were reduced in crown tissues of the grass-clump dwarf lines at the normal temperature. Unusual expression of the miR156/SPLs module could explain the grass-clump dwarf phenotype. Dramatic alteration of gene expression profiles, including miRNA levels, in crown tissues is associated with the temperature-dependent phenotypic plasticity in type II necrosis/grass-clump dwarf wheat hybrids.
Volume 12(5)
Pages e0176497
Published 2017-5-2
DOI 10.1371/journal.pone.0176497
PII PONE-D-16-49522
PMID 28463975
PMC PMC5413045
MeSH Crosses, Genetic Gene Expression Profiling MicroRNAs / genetics MicroRNAs / metabolism Poaceae / genetics* Poaceae / growth & development Polymerase Chain Reaction Temperature Tetraploidy Triticum / genetics* Triticum / growth & development
IF 2.74
Times Cited 3
Wheat KU-2012 KU-2025 KU-2059 KU-2075 KU-2159 KU-138