RRC ID 47985
著者 Ishikawa-Fujiwara T, Shiraishi E, Fujikawa Y, Mori T, Tsujimura T, Todo T.
タイトル Targeted Inactivation of DNA Photolyase Genes in Medaka Fish (Oryzias latipes).
ジャーナル Photochem Photobiol
Abstract Proteins of the cryptochrome/photolyase family (CPF) exhibit sequence and structural conservation, but their functions are divergent. Photolyase is a DNA repair enzyme that catalyzes the light-dependent repair of ultraviolet (UV)-induced photoproducts, whereas cryptochrome acts as a photoreceptor or circadian clock protein. Two types of DNA photolyase exist: CPD photolyase, which repairs cyclobutane pyrimidine dimers (CPDs), and 6-4 photolyase, which repairs 6-4 pyrimidine-pyrimidone photoproducts (6-4PPs). Although the Cry-DASH protein is classified as a cryptochrome, it also has light-dependent DNA repair activity. To determine the significance of the three light-dependent repair enzymes in recovering from solar UV-induced DNA damage at the organismal level, we generated mutants in each gene in medaka using the CRISPR genome editing technique. The light-dependent repair activity of the mutants was examined in vitro in cultured cells and in vivo in skin tissue. Light-dependent repair of CPD was lost in the CPD photolyase-deficient mutant, whereas weak repair activity against 6-4PPs persisted in the 6-4 photolyase-deficient mutant. These results suggest the existence of a heretofore unknown 6-4PP repair pathway and thus improve our understanding of the mechanisms of defense against solar UV in vertebrates.
巻・号 93(1)
ページ 315-322
公開日 2017-1-1
DOI 10.1111/php.12658
PMID 27861979
MeSH Animals Clustered Regularly Interspaced Short Palindromic Repeats Cryptochromes / metabolism DNA Damage DNA Repair Deoxyribodipyrimidine Photo-Lyase / genetics* Mutation* Oryzias / genetics* Pyrimidine Dimers / metabolism Ultraviolet Rays*
IF 2.721
引用数 2
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS
リソース情報
メダカ database (6-4)phr(del7) (MT1172) CPDphr(del5) (MT1173) cry-dash(del7) (MT1174)