論文 - 詳細
| RRC ID | 47985 |
|---|---|
| 著者 | Ishikawa-Fujiwara T, Shiraishi E, Fujikawa Y, Mori T, Tsujimura T, Todo T. |
| タイトル | Targeted Inactivation of DNA Photolyase Genes in Medaka Fish (Oryzias latipes). |
| ジャーナル | Photochem Photobiol |
| Abstract |
Proteins of the cryptochrome/photolyase family (CPF) exhibit sequence and structural conservation, but their functions are divergent. Photolyase is a DNA repair enzyme that catalyzes the light-dependent repair of ultraviolet (UV)-induced photoproducts, whereas cryptochrome acts as a photoreceptor or circadian clock protein. Two types of DNA photolyase exist: CPD photolyase, which repairs cyclobutane pyrimidine dimers (CPDs), and 6-4 photolyase, which repairs 6-4 pyrimidine-pyrimidone photoproducts (6-4PPs). Although the Cry-DASH protein is classified as a cryptochrome, it also has light-dependent DNA repair activity. To determine the significance of the three light-dependent repair enzymes in recovering from solar UV-induced DNA damage at the organismal level, we generated mutants in each gene in medaka using the CRISPR genome editing technique. The light-dependent repair activity of the mutants was examined in vitro in cultured cells and in vivo in skin tissue. Light-dependent repair of CPD was lost in the CPD photolyase-deficient mutant, whereas weak repair activity against 6-4PPs persisted in the 6-4 photolyase-deficient mutant. These results suggest the existence of a heretofore unknown 6-4PP repair pathway and thus improve our understanding of the mechanisms of defense against solar UV in vertebrates. |
| 巻・号 | 93(1) |
| ページ | 315-322 |
| 公開日 | 2017-1-1 |
| DOI | 10.1111/php.12658 |
| PMID | 27861979 |
| MeSH | Animals Clustered Regularly Interspaced Short Palindromic Repeats Cryptochromes / metabolism DNA Damage DNA Repair Deoxyribodipyrimidine Photo-Lyase / genetics* Mutation* Oryzias / genetics* Pyrimidine Dimers / metabolism Ultraviolet Rays* |
| IF | 2.721 |
| 引用数 | 2 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY BIOPHYSICS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| メダカ | database (6-4)phr(del7) (MT1172) CPDphr(del5) (MT1173) cry-dash(del7) (MT1174) |