RRC ID |
47985
|
著者 |
Ishikawa-Fujiwara T, Shiraishi E, Fujikawa Y, Mori T, Tsujimura T, Todo T.
|
タイトル |
Targeted Inactivation of DNA Photolyase Genes in Medaka Fish (Oryzias latipes).
|
ジャーナル |
Photochem Photobiol
|
Abstract |
Proteins of the cryptochrome/photolyase family (CPF) exhibit sequence and structural conservation, but their functions are divergent. Photolyase is a DNA repair enzyme that catalyzes the light-dependent repair of ultraviolet (UV)-induced photoproducts, whereas cryptochrome acts as a photoreceptor or circadian clock protein. Two types of DNA photolyase exist: CPD photolyase, which repairs cyclobutane pyrimidine dimers (CPDs), and 6-4 photolyase, which repairs 6-4 pyrimidine-pyrimidone photoproducts (6-4PPs). Although the Cry-DASH protein is classified as a cryptochrome, it also has light-dependent DNA repair activity. To determine the significance of the three light-dependent repair enzymes in recovering from solar UV-induced DNA damage at the organismal level, we generated mutants in each gene in medaka using the CRISPR genome editing technique. The light-dependent repair activity of the mutants was examined in vitro in cultured cells and in vivo in skin tissue. Light-dependent repair of CPD was lost in the CPD photolyase-deficient mutant, whereas weak repair activity against 6-4PPs persisted in the 6-4 photolyase-deficient mutant. These results suggest the existence of a heretofore unknown 6-4PP repair pathway and thus improve our understanding of the mechanisms of defense against solar UV in vertebrates.
|
巻・号 |
93(1)
|
ページ |
315-322
|
公開日 |
2017-1-1
|
DOI |
10.1111/php.12658
|
PMID |
27861979
|
MeSH |
Animals
Clustered Regularly Interspaced Short Palindromic Repeats
Cryptochromes / metabolism
DNA Damage
DNA Repair
Deoxyribodipyrimidine Photo-Lyase / genetics*
Mutation*
Oryzias / genetics*
Pyrimidine Dimers / metabolism
Ultraviolet Rays*
|
IF |
2.721
|
引用数 |
2
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
BIOPHYSICS
|
リソース情報 |
メダカ |
database
(6-4)phr(del7) (MT1172)
CPDphr(del5) (MT1173)
cry-dash(del7) (MT1174) |