RRC ID 48005
Author Kokusho R, Koh Y, Fujimoto M, Shimada T, Katsuma S.
Title Bombyx mori nucleopolyhedrovirus BM5 protein regulates progeny virus production and viral gene expression.
Journal Virology
Abstract Bombyx mori nucleopolyhedrovirus (BmNPV) orf5 (Bm5) is a core gene of lepidopteran baculoviruses and encodes the protein with the conserved amino acid residues (DUF3627) in its C-terminus. Here, we found that Bm5 disruption resulted in lower titers of budded viruses and fewer numbers of occlusion bodies (OBs) in B. mori cultured cells and larvae, although viral genome replication was not affected. Bm5 disruption also caused aberrant expression of various viral genes at the very late stage of infection. Immunocytochemical analysis revealed that BM5 localized to the nuclear membrane. We also found that DUF3627 is important for OB production, transcriptional regulation of viral genes, and subcellular localization of BM5. Compared with wild-type BmNPV infection, larval death was delayed when B. mori larvae were infected with Bm5 mutants. These results suggest that BM5 is involved in progeny virus production and regulation of viral gene expression at the very late stage of infection.
Volume 498
Pages 240-249
Published 2016-11-1
DOI 10.1016/j.virol.2016.08.032
PII S0042-6822(16)30248-3
PMID 27614700
MeSH Animals Bombyx / virology* Cell Line Cells, Cultured DNA Replication Gene Expression Regulation, Viral* Genome, Viral Host-Pathogen Interactions / genetics Intracellular Space Larva Mutation Nucleopolyhedroviruses / physiology* Protein Transport Recombinant Fusion Proteins Transcription, Genetic Viral Proteins / genetics Viral Proteins / metabolism* Virus Replication*
IF 2.819
Times Cited 4