RRC ID 4808
Author Wang YQ, Feechan A, Yun BW, Shafiei R, Hofmann A, Taylor P, Xue P, Yang FQ, Xie ZS, Pallas JA, Chu CC, Loake GJ.
Title S-nitrosylation of AtSABP3 antagonizes the expression of plant immunity.
Journal J. Biol. Chem.
Abstract Changes in cellular redox status are a well established response across phyla following pathogen challenge. In this context, the synthesis of nitric oxide (NO) is a conspicuous feature of plants responding to attempted microbial infection and this redox-based regulator underpins the development of plant immunity. However, the associated molecular mechanism(s) have not been defined. Here we show that NO accretion during the nitrosative burst promotes increasing S-nitrosylation of the Arabidopsis thaliana salicylic acid-binding protein 3 (AtSABP3) at cysteine (Cys) 280, suppressing both binding of the immune activator, salicylic acid (SA), and the carbonic anhydrase (CA) activity of this protein. The CA function of AtSABP3 is required for the expression of resistance in the host against attempted pathogen infection. Therefore, inhibition of AtSBAP3 CA function by S-nitrosylation could contribute to a negative feedback loop that modulates the plant defense response. Thus, AtSABP3 is one of the first targets for S-nitrosylation in plants for which the biological function of this redox-based post-translational modification has been uncovered. These data provide a molecular connection between the changes in NO levels triggered by attempted pathogen infection and the expression of disease resistance.
Volume 284(4)
Pages 2131-7
Published 2009-1-23
DOI 10.1074/jbc.M806782200
PII M806782200
PMID 19017644
MeSH Arabidopsis / genetics Arabidopsis / immunology* Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Carbonic Anhydrases / chemistry Carbonic Anhydrases / genetics Carbonic Anhydrases / metabolism* Gene Expression Regulation, Plant* Mass Spectrometry Models, Molecular Nitrogen Oxides / metabolism* Plant Diseases / genetics Plant Diseases / immunology Protein Binding Protein Multimerization Protein Structure, Quaternary Protein Structure, Tertiary
IF 4.011
Times Cited 109
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pda04430