RRC ID |
4808
|
著者 |
Wang YQ, Feechan A, Yun BW, Shafiei R, Hofmann A, Taylor P, Xue P, Yang FQ, Xie ZS, Pallas JA, Chu CC, Loake GJ.
|
タイトル |
S-nitrosylation of AtSABP3 antagonizes the expression of plant immunity.
|
ジャーナル |
J Biol Chem
|
Abstract |
Changes in cellular redox status are a well established response across phyla following pathogen challenge. In this context, the synthesis of nitric oxide (NO) is a conspicuous feature of plants responding to attempted microbial infection and this redox-based regulator underpins the development of plant immunity. However, the associated molecular mechanism(s) have not been defined. Here we show that NO accretion during the nitrosative burst promotes increasing S-nitrosylation of the Arabidopsis thaliana salicylic acid-binding protein 3 (AtSABP3) at cysteine (Cys) 280, suppressing both binding of the immune activator, salicylic acid (SA), and the carbonic anhydrase (CA) activity of this protein. The CA function of AtSABP3 is required for the expression of resistance in the host against attempted pathogen infection. Therefore, inhibition of AtSBAP3 CA function by S-nitrosylation could contribute to a negative feedback loop that modulates the plant defense response. Thus, AtSABP3 is one of the first targets for S-nitrosylation in plants for which the biological function of this redox-based post-translational modification has been uncovered. These data provide a molecular connection between the changes in NO levels triggered by attempted pathogen infection and the expression of disease resistance.
|
巻・号 |
284(4)
|
ページ |
2131-7
|
公開日 |
2009-1-23
|
DOI |
10.1074/jbc.M806782200
|
PII |
S0021-9258(19)81923-3
|
PMID |
19017644
|
MeSH |
Arabidopsis / genetics
Arabidopsis / immunology*
Arabidopsis / metabolism*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Carbonic Anhydrases / chemistry
Carbonic Anhydrases / genetics
Carbonic Anhydrases / metabolism*
Gene Expression Regulation, Plant*
Mass Spectrometry
Models, Molecular
Nitrogen Oxides / metabolism*
Plant Diseases / genetics
Plant Diseases / immunology
Protein Binding
Protein Multimerization
Protein Structure, Quaternary
Protein Structure, Tertiary
|
IF |
4.238
|
引用数 |
148
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda04430 |