RRC ID 48082
Author Ohta A, Shibuya I.
Title Membrane phospholipid synthesis and phenotypic correlation of an Escherichia coli pss mutant.
Journal J. Bacteriol.
Abstract A pair of putatively isogenic pss(Ts) and pss+ (phosphatidylserine synthetase structural gene) strains was constructed and analyzed, together with the revertants, for the physiological consequences of cessation of the optimal synthesis of phosphatidylethanolamine (PE). Their in vivo and in vitro abilities to synthetize PE and the growth rates at different temperatures were determined. The rate of PE synthesis by OS2101 pss(Ts) was inversely related to the culture temperature. OS2101 in a low-salt broth medium stopped division and formed filamentous cells with declining viability upon the elevation of culture temperature from 27 to 42 or 44 degrees C, whereas the syntheses of deoxyribonucleic acid, ribonucleic acid, and protein were not affected. Proper concentrations of cations such as Na+, K+, NH4+, and Mg2+ or of sucrose could remedy the division and growth of OS2101 at the restrictive temperature without restoring normal PE synthesis. A remedial effect other than osmotic protection of these effectors and an adaptive regulatory mechanism for PE formation are suggested.
Volume 132(2)
Pages 434-43
Published 1977-11
PMID 199578
PMC PMC221882
MeSH Bacterial Proteins / biosynthesis CDPdiacylglycerol-Serine O-Phosphatidyltransferase / genetics* CDPdiacylglycerol-Serine O-Phosphatidyltransferase / metabolism Carboxy-Lyases / metabolism DNA, Bacterial / biosynthesis Escherichia coli / enzymology Escherichia coli / genetics* Escherichia coli / metabolism Genes Magnesium / pharmacology Membrane Lipids / biosynthesis* Mutation* Osmotic Pressure Phenotype Phosphatidylethanolamines / biosynthesis Phospholipids / biosynthesis* Phosphotransferases / genetics* RNA, Bacterial / biosynthesis Sodium Chloride / pharmacology Sucrose / pharmacology Temperature
IF 3.234
Prokaryotes E. coli ME9790 ME9791 ME9792 ME9793