Reference - Detail
| RRC ID | 48093 |
|---|---|
| Author | Kawaguchi Y, Hoshimaru M, Nawa H, Nakanishi S. |
| Title | Sequence analysis of cloned cDNA for rat substance P precursor: existence of a third substance P precursor. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
The sequence of the mRNA for the rat substance P precursor (preprotachykinin A) has been elucidated by molecular cloning and sequence analysis. The deduced amino acid sequence of rat preprotachykinin A indicates that it contains both substance P and substance K but differs in the sequence organization from either bovine alpha- or beta-preprotachykinin A reported previously. The existence of the bovine mRNA for the third preprotachykinin A has thus been examined and evidenced by the isolation of the corresponding cDNA clone. This mRNA, named gamma-preprotachykinin A mRNA, deletes the sequence precisely corresponding to the exon 4 sequence of the preprotachykinin A gene. Thus, alternative RNA splicing in the expression of the single preprotachykinin A gene results in the generation of three different forms of the preprotachykinin A mRNAs. |
| Volume | 139(3) |
| Pages | 1040-6 |
| Published | 1986-9-30 |
| DOI | 10.1016/s0006-291x(86)80282-0 |
| PII | S0006-291X(86)80282-0 |
| PMID | 2429656 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular DNA / analysis* DNA Restriction Enzymes / metabolism Neuropeptides / genetics* Protein Precursors / genetics* RNA, Messenger / analysis Rats Substance P / biosynthesis* Tachykinins* |
| IF | 2.985 |
| Times Cited | 137 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| DNA material | pRSP 6 gamma PPT (RDB13175) |