| RRC ID |
48105
|
| 著者 |
Arai H, Hori S, Aramori I, Ohkubo H, Nakanishi S.
|
| タイトル |
Cloning and expression of a cDNA encoding an endothelin receptor.
|
| ジャーナル |
Nature
|
| Abstract |
Endothelins are a newly described peptide family consisting of three peptides (ET-1, ET-2 and ET-3) which are the most potent vasoconstrictive peptides known. They are crucial in the regulation of vascular smooth muscle tone. The diverse functions of endothelins are thought to be mediated by interaction with many different receptors coupled to the inositol phosphate/calcium ion messenger pathway. However, because of the structural resemblance of the three peptides, the presence and nature of multiple endothelin receptors remain to be elucidated. We report here the cloning of a complementary DNA encoding a bovine endothelin receptor, which has a transmembrane topology similar to that of other G protein-coupled receptors and shows specific binding, with the highest selectivity to ET-1 in animal cells transfected with the cloned cDNA. This receptor messenger RNA is widely distributed in the central nervous system and peripheral tissues, particularly in the heart and lung. Our results support the view that there are other receptor subtypes.
|
| 巻・号 |
348(6303)
|
| ページ |
730-2
|
| 公開日 |
1990-12-20
|
| DOI |
10.1038/348730a0
|
| PMID |
2175396
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Cattle
Cell Line
Cloning, Molecular*
DNA / genetics*
Endothelins / metabolism
Gene Expression*
Molecular Sequence Data
Nucleic Acid Hybridization
RNA, Messenger / analysis
RNA, Messenger / genetics
Receptors, Cell Surface / genetics*
Receptors, Cell Surface / metabolism
Receptors, Endothelin
Recombinant Proteins / metabolism
Sequence Homology, Nucleic Acid
Transfection
|
| IF |
42.779
|
| 引用数 |
2641
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
ETAR (RDB13191) |
