RRC ID 48108
Author Sugihara H, Moriyoshi K, Ishii T, Masu M, Nakanishi S.
Title Structures and properties of seven isoforms of the NMDA receptor generated by alternative splicing.
Journal Biochem Biophys Res Commun
Abstract We here report the existence of 6 additional isoforms of the NMDA receptor generated via alternative splicing by molecular analysis of cDNA clones isolated from a rat forebrain cDNA library. These isoforms possess the structures with an insertion at the extracellular amino-terminal region or deletions at two different extracellular carboxyl-terminal regions, or those formed by combinations of the above insertion and deletions. One of the deletions results in the generation of a new carboxyl-terminal sequence. All these isoforms possess the ability to induce electrophysiological responses to NMDA and respond to various antagonists selective to the NMDA receptor in the Xenopus oocyte expression system. In addition, a truncated form of the NMDA receptor also exists that contains only the extreme amino-terminal sequence of this protein molecule. These data indicate that the NMDA receptor consists of heterogeneous molecules that differ in the extracellular sequence of the amino- and carboxyl-terminal regions.
Volume 185(3)
Pages 826-32
Published 1992-6-30
DOI 10.1016/0006-291x(92)91701-q
PII 0006-291X(92)91701-Q
PMID 1352681
MeSH 2-Amino-5-phosphonovalerate / pharmacology Amino Acid Sequence Animals Base Sequence Chromosome Deletion* Cloning, Molecular DNA / genetics* DNA / isolation & purification Dizocilpine Maleate / pharmacology Gene Library Kynurenic Acid / pharmacology Molecular Sequence Data N-Methylaspartate / pharmacology Oocytes / drug effects Oocytes / physiology Piperazines / pharmacology Prosencephalon / physiology* RNA Splicing* RNA, Messenger / genetics* RNA, Messenger / metabolism Rats Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors Receptors, N-Methyl-D-Aspartate / genetics* Receptors, N-Methyl-D-Aspartate / physiology Restriction Mapping Xenopus
IF 2.985
Times Cited 502
DNA material R1 B - R1G (RDB13204)