| RRC ID |
48108
|
| 著者 |
Sugihara H, Moriyoshi K, Ishii T, Masu M, Nakanishi S.
|
| タイトル |
Structures and properties of seven isoforms of the NMDA receptor generated by alternative splicing.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
We here report the existence of 6 additional isoforms of the NMDA receptor generated via alternative splicing by molecular analysis of cDNA clones isolated from a rat forebrain cDNA library. These isoforms possess the structures with an insertion at the extracellular amino-terminal region or deletions at two different extracellular carboxyl-terminal regions, or those formed by combinations of the above insertion and deletions. One of the deletions results in the generation of a new carboxyl-terminal sequence. All these isoforms possess the ability to induce electrophysiological responses to NMDA and respond to various antagonists selective to the NMDA receptor in the Xenopus oocyte expression system. In addition, a truncated form of the NMDA receptor also exists that contains only the extreme amino-terminal sequence of this protein molecule. These data indicate that the NMDA receptor consists of heterogeneous molecules that differ in the extracellular sequence of the amino- and carboxyl-terminal regions.
|
| 巻・号 |
185(3)
|
| ページ |
826-32
|
| 公開日 |
1992-6-30
|
| DOI |
10.1016/0006-291x(92)91701-q
|
| PII |
0006-291X(92)91701-Q
|
| PMID |
1352681
|
| MeSH |
2-Amino-5-phosphonovalerate / pharmacology
Amino Acid Sequence
Animals
Base Sequence
Chromosome Deletion*
Cloning, Molecular
DNA / genetics*
DNA / isolation & purification
Dizocilpine Maleate / pharmacology
Gene Library
Kynurenic Acid / pharmacology
Molecular Sequence Data
N-Methylaspartate / pharmacology
Oocytes / drug effects
Oocytes / physiology
Piperazines / pharmacology
Prosencephalon / physiology*
RNA Splicing*
RNA, Messenger / genetics*
RNA, Messenger / metabolism
Rats
Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
Receptors, N-Methyl-D-Aspartate / genetics*
Receptors, N-Methyl-D-Aspartate / physiology
Restriction Mapping
Xenopus
|
| IF |
2.985
|
| 引用数 |
502
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 遺伝子材料 |
R1 B - R1G (RDB13204) |
