RRC ID 48125
著者 Pholsen S, Khota W, Pang H, Higgs D, Cai Y.
タイトル Characterization and application of lactic acid bacteria for tropical silage preparation.
ジャーナル Anim Sci J
Abstract Strains TH 14, TH 21 and TH 64 were isolated from tropical silages, namely corn stover, sugar cane top and rice straw, respectively, prepared in Thailand. These strains were selected by low pH growth range and high lactic acid-producing ability, similar to some commercial inoculants. Based on the analysis of 16S ribosomal RNA gene sequence and DNA-DNA relatedness, strain TH 14 was identified as Lactobacillus casei, and strains TH 21 and TH 64 were identified as L. plantarum. Strains TH 14, TH 21, TH 64 and two commercial inoculants, CH (L. plantarum) and SN (L. rhamnosus), were used as additives to fresh and wilted purple Guinea and sorghum silages prepared using a small-scale fermentation method. The number of epiphytic lactic acid bacteria (LAB) in the forages before ensilage was relatively low but the numbers of coliform and aerobic bacteria were higher. Sorghum silages at 30 days of fermentation were all well preserved with low pH (3.56) and high lactic acid production (72.86 g/kg dry matter). Purple Guinea silage inoculated with LAB exhibited reduced count levels of aerobic and coliform bacteria, lower pH, butyric acid and ammonia nitrogen and increased lactic acid concentration, compared with the control. Strain TH 14 more effectively improved lactic acid production compared with inoculants and other strains. © 2016 Japanese Society of Animal Science.
巻・号 87(10)
ページ 1202-1211
公開日 2016-10-1
DOI 10.1111/asj.12534
PMID 26799939
MeSH Fermentation Hydrogen-Ion Concentration Lactic Acid / biosynthesis Lactobacillus* / isolation & purification Lactobacillus* / metabolism Poaceae Silage / microbiology* Sorghum Thailand Tropical Climate
IF 1.399
引用数 19
WOS 分野 AGRICULTURE, DAIRY & ANIMAL SCIENCE
リソース情報
一般微生物 JCM 1149 JCM 1558 JCM 11302