論文 - 詳細
| RRC ID | 48173 |
|---|---|
| 著者 | McCarthy S, Johnson T, Pavlik BJ, Payne S, Schackwitz W, Martin J, Lipzen A, Keffeler E, Blum P. |
| タイトル | Expanding the Limits of Thermoacidophily in the Archaeon Sulfolobus solfataricus by Adaptive Evolution. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
Extremely thermoacidophilic Crenarchaeota belonging to the order Sulfolobales flourish in hot acidic habitats that are strongly oxidizing. The pH extremes of these habitats, however, often exceed the acid tolerance of type species and strains. Here, adaptive laboratory evolution was used over a 3-year period to test whether such organisms harbor additional thermoacidophilic capacity. Three distinct cell lines derived from a single type species were subjected to high-temperature serial passage while culture acidity was gradually increased. A 178-fold increase in thermoacidophily was achieved after 29 increments of shifted culture pH resulting in growth at pH 0.8 and 80°C. These strains were named super-acid-resistant Crenarchaeota (SARC). Mathematical modeling using growth parameters predicted the limits of acid resistance, while genome resequencing and transcriptome resequencing were conducted for insight into mechanisms responsible for the evolved trait. Among the mutations that were detected, a set of eight nonsynonymous changes may explain the heritability of increased acid resistance despite an unexpected lack of transposition. Four multigene components of the SARC transcriptome implicated oxidative stress as a primary challenge accompanying growth at acid extremes. These components included accelerated membrane biogenesis, induction of the mer operon, and an increased capacity for the generation of energy and reductant. |
| 巻・号 | 82(3) |
| ページ | 857-67 |
| 公開日 | 2016-2-1 |
| DOI | 10.1128/AEM.03225-15 |
| PII | AEM.03225-15 |
| PMID | 26590281 |
| PMC | PMC4725277 |
| MeSH | Adaptation, Physiological Archaeal Proteins / genetics Archaeal Proteins / metabolism Biotechnology Directed Molecular Evolution* Genome, Bacterial Hot Temperature* Hydrogen-Ion Concentration Models, Biological Multigene Family Mutation Operon Oxidation-Reduction Oxidative Stress / genetics Sequence Analysis, DNA Sulfolobus solfataricus / genetics* Sulfolobus solfataricus / growth & development Sulfolobus solfataricus / physiology* Time Factors Transcriptome |
| IF | 4.016 |
| 引用数 | 19 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 30930 |