論文 - 詳細
| RRC ID | 4823 |
|---|---|
| 著者 | Zeng CJ, Lee YR, Liu B. |
| タイトル | The WD40 repeat protein NEDD1 functions in microtubule organization during cell division in Arabidopsis thaliana. |
| ジャーナル | Plant Cell |
| Abstract |
Although cells of flowering plants lack a structurally defined microtubule-organizing center like the centrosome, organization of the spindles and phragmoplasts in mitosis is known to involve the evolutionarily conserved gamma-tubulin complex. We have investigated the function of Arabidopsis thaliana NEDD1, a WD40 repeat protein related to the animal NEDD1/GCP-WD protein, which interacts with the gamma-tubulin complex. The NEDD1 protein decorates spindle microtubules (MTs) preferentially toward spindle poles and phragmoplast MTs toward their minus ends. A T-DNA insertional allele of the single NEDD1 gene was isolated and maintained in heterozygous sporophytes, and NEDD1's function in cell division was analyzed in haploid microspores produced by the heterozygote. In approximately half of the dividing microspores exhibiting aberrant MT organization, spindles were no longer restricted to the cell periphery and became abnormally elongated. After mitosis, MTs aggregated between reforming nuclei but failed to appear in a bipolar configuration. Consequently, defective microspores did not form a continuous cell plate, and two identical nuclei were produced with no differentiation into generative and vegetative cells. Our results support the notion that the plant NEDD1 homolog plays a critical role in MT organization during mitosis, and its function is likely linked to that of the gamma-tubulin complex. |
| 巻・号 | 21(4) |
| ページ | 1129-40 |
| 公開日 | 2009-4-1 |
| DOI | 10.1105/tpc.109.065953 |
| PII | tpc.109.065953 |
| PMID | 19383896 |
| PMC | PMC2685624 |
| MeSH | Arabidopsis / cytology* Arabidopsis / metabolism Arabidopsis / ultrastructure Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Arabidopsis Proteins / physiology* Cell Division / physiology* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Microtubule-Associated Proteins / physiology* Microtubules / metabolism* Microtubules / physiology Microtubules / ultrastructure Mutagenesis, Insertional Phylogeny Spindle Apparatus / metabolism Spindle Apparatus / physiology Spindle Apparatus / ultrastructure |
| IF | 9.618 |
| 引用数 | 75 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pdz37649 |