RRC ID 4823
著者 Zeng CJ, Lee YR, Liu B.
タイトル The WD40 repeat protein NEDD1 functions in microtubule organization during cell division in Arabidopsis thaliana.
ジャーナル Plant Cell
Abstract Although cells of flowering plants lack a structurally defined microtubule-organizing center like the centrosome, organization of the spindles and phragmoplasts in mitosis is known to involve the evolutionarily conserved gamma-tubulin complex. We have investigated the function of Arabidopsis thaliana NEDD1, a WD40 repeat protein related to the animal NEDD1/GCP-WD protein, which interacts with the gamma-tubulin complex. The NEDD1 protein decorates spindle microtubules (MTs) preferentially toward spindle poles and phragmoplast MTs toward their minus ends. A T-DNA insertional allele of the single NEDD1 gene was isolated and maintained in heterozygous sporophytes, and NEDD1's function in cell division was analyzed in haploid microspores produced by the heterozygote. In approximately half of the dividing microspores exhibiting aberrant MT organization, spindles were no longer restricted to the cell periphery and became abnormally elongated. After mitosis, MTs aggregated between reforming nuclei but failed to appear in a bipolar configuration. Consequently, defective microspores did not form a continuous cell plate, and two identical nuclei were produced with no differentiation into generative and vegetative cells. Our results support the notion that the plant NEDD1 homolog plays a critical role in MT organization during mitosis, and its function is likely linked to that of the gamma-tubulin complex.
巻・号 21(4)
ページ 1129-40
公開日 2009-4-1
DOI 10.1105/tpc.109.065953
PII tpc.109.065953
PMID 19383896
PMC PMC2685624
MeSH Arabidopsis / cytology* Arabidopsis / metabolism Arabidopsis / ultrastructure Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Arabidopsis Proteins / physiology* Cell Division / physiology* Microtubule-Associated Proteins / genetics Microtubule-Associated Proteins / metabolism Microtubule-Associated Proteins / physiology* Microtubules / metabolism* Microtubules / physiology Microtubules / ultrastructure Mutagenesis, Insertional Phylogeny Spindle Apparatus / metabolism Spindle Apparatus / physiology Spindle Apparatus / ultrastructure
IF 9.618
引用数 75
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pdz37649