Reference - Detail
| RRC ID | 4835 |
|---|---|
| Author | Jin H, Hong Z, Su W, Li J. |
| Title | A plant-specific calreticulin is a key retention factor for a defective brassinosteroid receptor in the endoplasmic reticulum. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Mammalian calreticulin (CRT) is a multifunctional Ca(2+)-binding protein involved in more than 40 cellular processes in various subcellular compartments, such as Ca(2+) storage and protein folding in the endoplasmic reticulum (ER). CRT homologues were discovered in plants almost 15 years ago, and recent studies revealed that many plant species contain 2 or more CRTs that are members of 2 distinct families, the CRT1/2 family and the plant-specific CRT3 family. However, little is known about their physiological functions. Here we report ebs2 (EMS-mutagenized bri1 suppressor 2) as an allele-specific suppressor of bri1-9, a dwarf Arabidopsis mutant caused by retention of a defective brassinosteroid receptor in the ER. EBS2 encodes the Arabidopsis CRT3 that interacts with ER-localized bri1-9 in a glycan-dependent manner. Loss-of-function ebs2 mutations compromise ER retention of bri1-9 and suppress its dwarfism, whereas EBS2 over-expression enhances its dwarf phenotype. In contrast, mutations of 2 other CRTs or their membrane-localized homologues calnexins had little effect on bri1-9. A domain-swapping experiment revealed that the positively charged C-terminal tail of CRT3 is crucial for its "bri1-9-retainer" function. Our study revealed not only a functional role for a plant-specific CRT, but also functional diversity among the 3 Arabidopsis CRT paralogues. |
| Volume | 106(32) |
| Pages | 13612-7 |
| Published | 2009-8-11 |
| DOI | 10.1073/pnas.0906144106 |
| PII | 0906144106 |
| PMID | 19597144 |
| PMC | PMC2726391 |
| MeSH | Alleles Amino Acid Sequence Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Brassinosteroids Calreticulin / chemistry Calreticulin / genetics Calreticulin / metabolism* Cholestanols / metabolism* Cloning, Molecular Endoplasmic Reticulum / metabolism* Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Mutation / genetics Phenotype Plant Lectins / metabolism Protein Binding Receptors, Cell Surface / metabolism* Species Specificity Steroids, Heterocyclic / metabolism* Suppression, Genetic |
| IF | 9.412 |
| Times Cited | 80 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda04763 pda12372 |