| RRC ID |
48431
|
| Author |
Zhao J, Guo L, Liu C, Zhang Y, Guan X, Li J, Xu S, Xiang W, Wang X.
|
| Title |
Micromonospora lycii sp. nov., a novel endophytic actinomycete isolated from wolfberry root (Lycium chinense Mill).
|
| Journal |
J Antibiot (Tokyo)
|
| Abstract |
A novel actinomycete, designated strain NEAU-gq11(T), was isolated from wolfberry root (Lycium chinense Mill) and characterized using a polyphasic approach. Strain NEAU-gq11(T) was observed to form rough-surfaced spores that borne singly on the substrate hyphae but aerial mycelia were not developed. The organism showed closest 16S rRNA gene sequence similarity to Micromonospora saelicesensis Lupac 09(T) (99.4%), and phylogenetically clustered with M. chokoriensis 2-19/6(T) (99.3%), 'M. zeae' NEAU-gq9(T) (99.3%), M. violae NEAU-zh8(T) (99.1%), 'M. jinlongensis' NEAU-GRX11(T) (99.1%), M. zamorensis CR38(T) (99.0%), M. taraxaci NEAU-P5(T) (98.9%) and M. lupini Lupac 14N(T) (98.6%). Phylogenetic analysis based on the gyrB gene sequences also indicated that the isolate clustered with the above type strains except M. violae NEAU-zh8(T). The cell-wall peptidoglycan consisted of meso-diaminopimelic acid and glycine. The major menaquinones were MK-9(H8), MK-10(H6) and MK-10(H2). The phospholipid profile contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acid was iso-C16:0. Furthermore, some physiological and biochemical properties and low DNA-DNA relatedness values enabled the strain to be differentiated from members of closely related species. Therefore, it is proposed that strain NEAU-gq11(T) represents a novel species of the genus Micromonospora, for which the name Micromonospora lycii sp. nov. is proposed. The type strain is NEAU-gq11(T) (=CGMCC 4.7149(T)=DSM 46703(T)).
|
| Volume |
69(3)
|
| Pages |
153-8
|
| Published |
2016-3-1
|
| DOI |
10.1038/ja.2015.109
|
| PII |
ja2015109
|
| PMID |
26506858
|
| MeSH |
Bacterial Typing Techniques
Cell Wall / chemistry
DNA, Bacterial / genetics
Diaminopimelic Acid / chemistry
Fatty Acids / chemistry
Genes, Bacterial*
Glycine / chemistry
Lycium / microbiology*
Micromonospora / classification*
Micromonospora / genetics
Micromonospora / isolation & purification
Peptidoglycan / chemistry
Phospholipids / analysis
Phylogeny*
Plant Roots / microbiology*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Vitamin K 2 / chemistry
|
| IF |
2.668
|
| Times Cited |
7
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
IMMUNOLOGY
|
| Resource |
| General Microbes |
JCM 16032
JCM 13247
JCM 16031 |
