| RRC ID |
48544
|
| 著者 |
Kobayashi T, Agustini TW, Ibrahim R, Kamei K, Kondo A, Kajiwara M, Ooka Y, Nakamura H, Terahara T, Imada C.
|
| タイトル |
Production of bacteriocin by Virgibacillus salexigens isolated from "terasi": a traditionally fermented shrimp paste in Indonesia.
|
| ジャーナル |
World J Microbiol Biotechnol
|
| Abstract |
A natural antibacterial-substance-producing gram-positive bacterium was isolated from terasi shrimp paste, a popular fermented product in Indonesia. This strain, a spore-forming and strictly aerobic bacterium, was identified as Virgibacillus salexigens by 16S rRNA gene sequence analysis. The antibacterial substance purified from the precipitated product in the culture supernatant of the strain using ammonium sulfate showed a broad inhibition spectrum against gram-positive bacteria, including a typical foodborne bacterium, namely, Listeria monocytogenes. The antibacterial activity of the substance was inactivated by treatments with various proteolytic enzymes. It was stable after heating or pH treatment, and approximately 60% of the initial activity remained even after heating at 121 °C for 15 min. In addition, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis indicated that its monoisotopic mass weight was 5318.4 Da (M+H)(+). On the basis of the results obtained by the automated Edman degradation technique and MALDI-TOF MS analysis, the substance can be classified as a member of Class IId bacteriocins, but it could not be identified as any of the previously purified substances except for the putative bacteriocin predicted from the draft genome sequence data of gram-positive bacteria such as Virgibacillus and Bacillus strains.
|
| 巻・号 |
32(3)
|
| ページ |
47
|
| 公開日 |
2016-3-1
|
| DOI |
10.1007/s11274-015-1991-2
|
| PII |
10.1007/s11274-015-1991-2
|
| PMID |
26873558
|
| MeSH |
Aerobiosis
Bacteriocins / isolation & purification
Bacteriocins / metabolism*
Bacteriocins / pharmacology*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Food Microbiology*
Gram-Positive Bacteria / drug effects*
Hydrogen-Ion Concentration
Indonesia
Molecular Weight
Proteolysis
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spores, Bacterial / cytology
Temperature
Virgibacillus / classification
Virgibacillus / genetics
Virgibacillus / isolation & purification*
Virgibacillus / metabolism*
|
| IF |
2.477
|
| 引用数 |
3
|
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| 一般微生物 |
JCM 5803
JCM 1149
JCM 1649 |
