Reference - Detail
|Author||Mita K, Fujiwara S.|
|Title||Nodal regulates neural tube formation in the Ciona intestinalis embryo.|
|Journal||Dev. Genes Evol.|
Overexpression of a lefty orthologue, Ci-lefty, caused a failure of neural tube closure in the protochordate ascidian Ciona intestinalis. The body bent dorsally, and anterior-posterior elongation was inhibited. A similar phenotype was observed in embryos treated with SB431542, an inhibitor of Nodal receptors, suggesting that Ci-Lefty antagonized Nodal signaling as reported in other deuterostome species. Overexpression of Ci-nodal also resulted in a similar phenotype, suggesting that a correct quantity and/or a spatial restriction of Nodal signaling are important for the neural tube to form. In addition to known Ci-Nodal target genes, orthologues of Zic (Ci-ZicL) and cdx (Ci-cdx) were activated by Ci-Nodal. Expression of a dominant negative Ci-cdx caused defects in neural tube formation similar to those obtained on treatment with SB431542 or overexpression of Ci-lefty. A regulatory cascade composed of Ci-Nodal, Ci-ZicL, and Ci-Cdx may play an important role in neural tube formation in the Ciona embryo.
|MeSH||Animals Animals, Genetically Modified Base Sequence Ciona intestinalis / embryology* Ciona intestinalis / genetics* Embryo, Nonmammalian Embryonic Development / genetics* Gene Expression Profiling Gene Expression Regulation, Developmental Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Left-Right Determination Factors Models, Biological Molecular Sequence Data Neural Tube / growth & development* Neural Tube / metabolism Nodal Protein Sequence Homology, Nucleic Acid Transfection Transforming Growth Factor beta / genetics Transforming Growth Factor beta / physiology*|
|WOS Category||EVOLUTIONARY BIOLOGY DEVELOPMENTAL BIOLOGY CELL BIOLOGY|
|C.intestinalis / (O.japonicus)||Wild C.int|