論文 - 詳細
| RRC ID | 48751 |
|---|---|
| 著者 | Vu KV, Nguyen NT, Jeong CY, Lee YH, Lee H, Hong SW. |
| タイトル | Systematic deletion of the ER lectin chaperone genes reveals their roles in vegetative growth and male gametophyte development in Arabidopsis. |
| ジャーナル | Plant J |
| Abstract |
Calnexin (CNX) and calreticulin (CRT) are homologous lectin chaperones in the endoplasmic reticulum (ER) that facilitate glycoprotein folding and retain folding intermediates to prevent their transit via the secretary pathway. The Arabidopsis genome has two CNX (CNX1 and CNX2) and three CRT (CRT1, CRT2 and CRT3) homologs. Despite growing evidence of the biological roles of CNXs and CRTs, little is understood about their function in Arabidopsis growth and development under normal conditions. Here, we report that the deletion of CNX1, but not of CNX2, in the crt1 crt2 crt3 triple mutation background had an adverse effect on pollen viability and pollen tube growth, leading to a significant reduction in fertility. The cnx1 crt1 crt2 crt3 quadruple mutation also conferred severe defects in growth and development, including a shortened primary root, increased root hair length and density, and reduced plant height. Disruption of all five members of the CNX/CRT family was revealed to be lethal. Finally, the abnormal phenotype of the cnx1 crt1 crt2 crt3 quadruple mutants was completely rescued by either the CNX1 or CNX2 cDNA under the control of the CNX1 promoter, suggesting functional redundancy between CNX1 and CNX2. Taken together, these results provide genetic evidence that CNX and CRT play essential and overlapping roles during vegetative growth and male gametophyte development in Arabidopsis. |
| 巻・号 | 89(5) |
| ページ | 972-983 |
| 公開日 | 2017-3-1 |
| DOI | 10.1111/tpj.13435 |
| PMID | 27888524 |
| MeSH | Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Calnexin / genetics Calnexin / metabolism Calreticulin / genetics Calreticulin / metabolism DNA, Complementary / metabolism Endodeoxyribonucleases / genetics Endodeoxyribonucleases / metabolism Endoplasmic Reticulum / metabolism* Lectins / metabolism* Protein Folding |
| IF | 6.141 |
| 引用数 | 8 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda12225 pda04013 |