RRC ID 48765
Author Plante S, Normant V, Ramos-Torres KM, Labbé S.
Title Cell-surface copper transporters and superoxide dismutase 1 are essential for outgrowth during fungal spore germination.
Journal J. Biol. Chem.
Abstract deletion only partially interferes with formation of outgrowing spores. At outgrowth, Ctr4-GFP and Ctr5-Cherry first co-localize at the spore contour, followed by re-location to a middle peripheral spore region. Subsequently, they move away from the spore body to occupy the periphery of the nascent cell. After breaking of spore dormancy, Ctr6 localizes to the vacuole membranes that are enriched in the spore body relative to the germ tube. Using a copper-binding tracker, results showed that labile copper is preferentially localized to the spore body. Further analysis showed that Ctr4 and Ctr6 are required for copper-dependent activation of the superoxide dismutase 1 (SOD1) during spore germination. This activation is critical because the loss of SOD1 activity blocked spore germination at outgrowth. Taken together, these results indicate that cell-surface copper transporters and SOD1 are required for completion of the spore germination program.
Volume 292(28)
Pages 11896-11914
Published 2017-7-14
DOI 10.1074/jbc.M117.794677
PII M117.794677
PMID 28572514
PMC PMC5512082
MeSH Cation Transport Proteins / genetics Cation Transport Proteins / metabolism* Copper / metabolism Enzyme Activation Gene Deletion Gene Expression Regulation, Fungal* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Luminescent Proteins / genetics Luminescent Proteins / metabolism Microscopy, Fluorescence Microscopy, Interference Microscopy, Phase-Contrast Protein Isoforms / genetics Protein Isoforms / metabolism Protein Transport RNA, Fungal / metabolism RNA, Messenger / metabolism Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Schizosaccharomyces / cytology Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Spores, Fungal / cytology Spores, Fungal / growth & development Spores, Fungal / metabolism Spores, Fungal / physiology* Superoxide Dismutase-1 / metabolism* Transcription Factors / genetics Transcription Factors / metabolism*
IF 4.011
Yeast FY12806