論文 - 詳細
| RRC ID | 48812 |
|---|---|
| 著者 | Miwa D, Sakaue T, Inoue H, Takemori N, Kurokawa M, Fukuda S, Omi K, Goishi K, Higashiyama S. |
| タイトル | Protein kinase D2 and heat shock protein 90 beta are required for BCL6-associated zinc finger protein mRNA stabilization induced by vascular endothelial growth factor-A. |
| ジャーナル | Angiogenesis |
| Abstract |
Vascular endothelial growth factor (VEGF) is a major angiogenic factor that activates pro-angiogenic molecules to generate new vessels. Recently, we identified a VEGF-A-induced pro-angiogenic gene, BCL-6 associated zinc finger protein (BAZF), in endothelial cells. BAZF interacts with CBF1, a transcriptional regulator of Notch signaling, and downregulates Notch signaling by inducing the degradation of CBF1. A signal inhibition assay with a combination of chemical inhibitors and siRNA revealed that the protein kinase D (PRKD) family, mainly PRKD2, mediated BAZF gene expression by VEGF-A stimulation. A luciferase reporter assay showed that the promoter activity of the BAZF gene was unchanged by VEGF-A stimulation. However, we found that the stability of BAZF mRNA increased in a VEGF-A/PRKD2-dependent manner. In further studies to investigate the underlying mechanism, we successfully identified heat shock protein 90 beta (HSP90β) as a molecule that interacts with and stabilizes BAZF mRNA following VEGF-A/PRKD2 activation. These data suggest that HSP90β may positively regulate angiogenesis, not only as a protein chaperone, but also as an mRNA stabilizer for pro-angiogenic genes, such as BAZF, in a PRKD2 activity-dependent manner. |
| 巻・号 | 16(3) |
| ページ | 675-88 |
| 公開日 | 2013-7-1 |
| DOI | 10.1007/s10456-013-9345-x |
| PMID | 23515950 |
| MeSH | Blotting, Western Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation / physiology* HSP90 Heat-Shock Proteins / metabolism* Human Umbilical Vein Endothelial Cells Humans Immunoglobulin J Recombination Signal Sequence-Binding Protein / metabolism Luciferases Protein Kinase C / metabolism Protein Kinase D2 Protein Kinases / metabolism* RNA Interference RNA Stability / drug effects RNA Stability / physiology* Real-Time Polymerase Chain Reaction Receptors, Notch / metabolism Repressor Proteins / metabolism Repressor Proteins / physiology* Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / drug effects Vascular Endothelial Growth Factor A / pharmacology |
| IF | 9.78 |
| 引用数 | 11 |
| WOS 分野 | PERIPHERAL VASCULAR DISEASE |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-CMV-MCS-IRES2-Bsd (RDB04385) |