RRC ID 48815
Author Tashiro K, Tada H, Heilker R, Shirozu M, Nakano T, Honjo T.
Title Signal sequence trap: a cloning strategy for secreted proteins and type I membrane proteins.
Journal Science
Abstract A method was developed to clone, without the use of specific functional assays, complementary DNAs (cDNAs) that carry specific amino-terminal signal sequences, such as those encoding intercellular signal-transducing molecules and receptors. The vector used in this system directed the cell surface expression of interleukin-2 receptor fusion proteins when inserts with signal sequences were cloned in-frame with the correct orientation. An expression cDNA library was constructed from a bone marrow stromal cell line, which contained 5' portion-enriched cDNAs (the average size was 400 base pairs). Two cDNAs that encoded putative cytokine molecules, stromal cell-derived factor-1 alpha (SDF-1 alpha) and SDF-1 beta, which belong to the intercrine-macrophage inflammatory protein superfamily, were cloned.
Volume 261(5121)
Pages 600-3
Published 1993-7-30
DOI 10.1126/science.8342023
PMID 8342023
MeSH Amino Acid Sequence Animals Base Sequence Cell Line Chemokine CXCL12 Chemokines, CXC* Cloning, Molecular / methods* Cytokines / chemistry Cytokines / genetics* Genetic Vectors Molecular Sequence Data Open Reading Frames Protein Sorting Signals / chemistry Protein Sorting Signals / genetics* Protein Sorting Signals / metabolism Receptors, Interleukin-2 / genetics Recombinant Fusion Proteins / biosynthesis Transfection
IF 41.846
Times Cited 611
DNA material mouse SDF-1 alpha cDNA (RDB06778) mouse SDF-1 beta cDNA (RDB06779).