RRC ID |
48817
|
著者 |
Shirozu M, Tada H, Tashiro K, Nakamura T, Lopez ND, Nazarea M, Hamada T, Sato T, Nakano T, Honjo T.
|
タイトル |
Characterization of novel secreted and membrane proteins isolated by the signal sequence trap method.
|
ジャーナル |
Genomics
|
Abstract |
We recently described a method, called the signal sequence trap (SST) method, to clone cDNAs of secreted proteins and/or type I transmembrane proteins containing N-terminal signal sequences by using an epitope-tagging expression plasmid vector. In this paper we describe the summary of a large-scale screening of approximately 5900 clones of an SST cDNA library constructed from mouse bone marrow stromal cell line ST-2 cells. Of 26 positive clones obtained and sequenced, 11 clones appeared to contain authentic signal sequences. Five of the clones corresponded to the 5' ends of the cDNA of known genes containing N-terminal signal sequences. The full-length cDNA clones of the 6 other unknown clones were isolated and sequenced. One clone, termed SDF3, encoded a mouse counterpart of human pigment epithelium-derived factor. Another clone, termed SDR1, had considerable homology with basigin, a member of the immunoglobulin superfamily. A third clone, termed SDF5, had partial homology with a Drosophila tissue polarity gene frizzled (fz) and its rat homologues, fz-1 and fz-2. The other three clones had no significant homology with sequences in the databases. These results indicate that the SST method is effective and useful for the isolation of secreted and membrane proteins without knowledge of their functions.
|
巻・号 |
37(3)
|
ページ |
273-80
|
公開日 |
1996-11-1
|
DOI |
10.1006/geno.1996.0560
|
PII |
S0888-7543(96)90560-1
|
PMID |
8938438
|
MeSH |
Amino Acid Sequence
Animals
Antigens, CD*
Antigens, Neoplasm*
Antigens, Surface*
Avian Proteins*
Basigin
Blood Proteins*
Bone Marrow / chemistry
Cloning, Molecular / methods*
DNA, Complementary / genetics*
Drosophila Proteins*
Drosophila melanogaster / genetics
Frizzled Receptors
Gene Library
Humans
Male
Membrane Glycoproteins / chemistry
Membrane Proteins / chemistry
Membrane Proteins / genetics
Membrane Proteins / isolation & purification*
Membrane Proteins / metabolism
Mice
Molecular Sequence Data
Rats
Receptors, G-Protein-Coupled
Sequence Alignment
Sequence Homology, Amino Acid
Signal Transduction*
Species Specificity
|
IF |
6.205
|
引用数 |
68
|
WOS 分野
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
GENETICS & HEREDITY
|
リソース情報 |
遺伝子材料 |
mouse SDF-5 cDNA (RDB06782)
mouse SDR-1 alpha cDNA (RDB06783)
mouse SDR-1 beta cDNA (RDB06784). |