論文 - 詳細
| RRC ID | 48860 |
|---|---|
| 著者 | Lim NR, Shohayeb B, Zaytseva O, Mitchell N, Millard SS, Ng DCH, Quinn LM. |
| タイトル | Glial-Specific Functions of Microcephaly Protein WDR62 and Interaction with the Mitotic Kinase AURKA Are Essential for Drosophila Brain Growth. |
| ジャーナル | Stem Cell Reports |
| Abstract |
The second most commonly mutated gene in primary microcephaly (MCPH) patients is wd40-repeat protein 62 (wdr62), but the relative contribution of WDR62 function to the growth of major brain lineages is unknown. Here, we use Drosophila models to dissect lineage-specific WDR62 function(s). Interestingly, although neural stem cell (neuroblast)-specific depletion of WDR62 significantly decreased neuroblast number, brain size was unchanged. In contrast, glial lineage-specific WDR62 depletion significantly decreased brain volume. Moreover, loss of function in glia not only decreased the glial population but also non-autonomously caused neuroblast loss. We further demonstrated that WDR62 controls brain growth through lineage-specific interactions with master mitotic signaling kinase, AURKA. Depletion of AURKA in neuroblasts drives brain overgrowth, which was suppressed by WDR62 co-depletion. In contrast, glial-specific depletion of AURKA significantly decreased brain volume, which was further decreased by WDR62 co-depletion. Thus, dissecting relative contributions of MCPH factors to individual neural lineages will be critical for understanding complex diseases such as microcephaly. |
| 巻・号 | 9(1) |
| ページ | 32-41 |
| 公開日 | 2017-7-11 |
| DOI | 10.1016/j.stemcr.2017.05.015 |
| PII | S2213-6711(17)30222-9 |
| PMID | 28625535 |
| PMC | PMC5511370 |
| MeSH | Animals Aurora Kinase A / genetics Aurora Kinase A / metabolism* Brain / growth & development* Brain / metabolism Drosophila / genetics Drosophila / growth & development* Drosophila / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Gene Knockdown Techniques Mitosis Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Neuroglia / cytology Neuroglia / metabolism* Protein Interaction Maps* |
| IF | 6.032 |
| 引用数 | 15 |
| WOS 分野 | CELL & TISSUE ENGINEERING CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 7337-R1 |