RRC ID 4912
著者 Inoue T, Shingaki R, Hirose S, Waki K, Mori H, Fukui K.
タイトル Genome-wide screening of genes required for swarming motility in Escherichia coli K-12.
ジャーナル J Bacteriol
Abstract Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type 1 fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain.
巻・号 189(3)
ページ 950-7
公開日 2007-2-1
DOI 10.1128/JB.01294-06
PII JB.01294-06
PMID 17122336
PMC PMC1797309
MeSH Antigens, Bacterial / genetics Antigens, Bacterial / physiology Citric Acid Cycle / genetics Citric Acid Cycle / physiology Escherichia coli / genetics* Escherichia coli / physiology Escherichia coli / ultrastructure Escherichia coli Proteins / chemistry Escherichia coli Proteins / genetics* Escherichia coli Proteins / physiology Fimbriae, Bacterial / chemistry Fimbriae, Bacterial / genetics Fimbriae, Bacterial / physiology Gene Deletion Genes, Bacterial* Genome, Bacterial* Glucose / metabolism Microscopy, Electron, Transmission Molecular Chaperones / genetics Molecular Chaperones / physiology Polysaccharides, Bacterial / metabolism Protein Folding Signal Transduction / genetics Signal Transduction / physiology
IF 3.006
引用数 122
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) Keno collection