Reference - Detail
|Author||Otani T, Ogura Y, Misaki K, Maeda T, Kimpara A, Yonemura S, Hayashi S.|
|Title||IKKε inhibits PKC to promote Fascin-dependent actin bundling.|
Signaling molecules have pleiotropic functions and are activated by various extracellular stimuli. Protein kinase C (PKC) is activated by diverse receptors, and its dysregulation is associated with diseases including cancer. However, how the undesired activation of PKC is prevented during development remains poorly understood. We have previously shown that a protein kinase, IKKε, is active at the growing bristle tip and regulates actin bundle organization during Drosophila bristle morphogenesis. Here, we demonstrate that IKKε regulates the actin bundle localization of a dynamic actin cross-linker, Fascin. IKKε inhibits PKC, thereby protecting Fascin from inhibitory phosphorylation. Excess PKC activation is responsible for the actin bundle defects in IKKε-deficient bristles, whereas PKC is dispensable for bristle morphogenesis in wild-type bristles, indicating that PKC is repressed by IKKε in wild-type bristle cells. These results suggest that IKKε prevents excess activation of PKC during bristle morphogenesis.
|MeSH||Actins / genetics Actins / metabolism* Animals Carrier Proteins / genetics Carrier Proteins / metabolism* Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism* I-kappa B Kinase / genetics I-kappa B Kinase / metabolism Microfilament Proteins / genetics Microfilament Proteins / metabolism* Phosphorylation Protein Kinase C / genetics Protein Kinase C / metabolism* Signal Transduction|
|WOS Category||DEVELOPMENTAL BIOLOGY|
|DNA material||pUAST-PKC53E-EGFP (RDB15213) pUAST-IKKe-GFP (RDB15216) pUAST-IKKe-IRES-mKO (RDB15217) pUAST-IKKe[K41A]-myc (RDB15219) pUAST-IKKe[K41A]-GFP (RDB15220) pUAST-IKKe[K41A]-IRES-mKO (RDB15221).|