RRC ID 49313
Author Ezura K, Ji-Seong K, Mori K, Suzuki Y, Kuhara S, Ariizumi T, Ezura H.
Title Genome-wide identification of pistil-specific genes expressed during fruit set initiation in tomato (Solanum lycopersicum).
Journal PLoS One
Abstract Fruit set involves the developmental transition of an unfertilized quiescent ovary in the pistil into a fruit. While fruit set is known to involve the activation of signals (including various plant hormones) in the ovary, many biological aspects of this process remain elusive. To further expand our understanding of this process, we identified genes that are specifically expressed in tomato (Solanum lycopersicum L.) pistils during fruit set through comprehensive RNA-seq-based transcriptome analysis using 17 different tissues including pistils at six different developmental stages. First, we identified 532 candidate genes that are preferentially expressed in the pistil based on their tissue-specific expression profiles. Next, we compared our RNA-seq data with publically available transcriptome data, further refining the candidate genes that are specifically expressed within the pistil. As a result, 108 pistil-specific genes were identified, including several transcription factor genes that function in reproductive development. We also identified genes encoding hormone-like peptides with a secretion signal and cysteine-rich residues that are conserved among some Solanaceae species, suggesting that peptide hormones may function as signaling molecules during fruit set initiation. This study provides important information about pistil-specific genes, which may play specific roles in regulating pistil development in relation to fruit set.
Volume 12(7)
Pages e0180003
Published 2017-7-6
DOI 10.1371/journal.pone.0180003
PII PONE-D-17-08655
PMID 28683065
PMC PMC5500324
MeSH Flowers / genetics* Flowers / growth & development Flowers / metabolism Fruit / genetics* Fruit / growth & development Fruit / metabolism Gene Expression Profiling Gene Expression Regulation, Plant* Gene Ontology Genome, Plant* High-Throughput Nucleotide Sequencing Lycopersicon esculentum / genetics* Lycopersicon esculentum / growth & development Lycopersicon esculentum / metabolism Molecular Sequence Annotation Organ Specificity Plant Growth Regulators / genetics Plant Growth Regulators / metabolism Plant Proteins / genetics* Plant Proteins / metabolism Signal Transduction Transcription Factors / genetics Transcription Factors / metabolism Transcriptome*
IF 2.74
Times Cited 13
Tomato TOMJPF00001