論文 - 詳細
| RRC ID | 49348 |
|---|---|
| 著者 | Sasaki T, Sato Y, Higashiyama T, Sasaki N. |
| タイトル | Live imaging reveals the dynamics and regulation of mitochondrial nucleoids during the cell cycle in Fucci2-HeLa cells. |
| ジャーナル | Sci Rep |
| Abstract |
Mitochondrial DNA (mtDNA) is organized in nucleoprotein complexes called mitochondrial nucleoids (mt-nucleoids), which are critical units of mtDNA replication and transmission. In humans, several hundreds of mt-nucleoids exist in a cell. However, how numerous mt-nucleoids are maintained during the cell cycle remains elusive, because cell cycle synchronization procedures affect mtDNA replication. Here, we analyzed regulation of the maintenance of mt-nucleoids in the cell cycle, using a fluorescent cell cycle indicator, Fucci2. Live imaging of mt-nucleoids with higher temporal resolution showed frequent attachment and detachment of mt-nucleoids throughout the cell cycle. TFAM, an mtDNA packaging protein, was involved in the regulation of this dynamic process, which was important for maintaining proper mt-nucleoid number. Both an increase in mt-nucleoid number and activation of mtDNA replication occurred during S phase. To increase mt-nucleoid number, mtDNA replication, but not nuclear DNA replication, was necessary. We propose that these dynamic and regulatory processes in the cell cycle maintain several hundred mt-nucleoids in proliferating cells. |
| 巻・号 | 7(1) |
| ページ | 11257 |
| 公開日 | 2017-9-12 |
| DOI | 10.1038/s41598-017-10843-8 |
| PII | 10.1038/s41598-017-10843-8 |
| PMID | 28900194 |
| PMC | PMC5595809 |
| MeSH | Cell Cycle* DNA, Mitochondrial / metabolism* DNA-Binding Proteins / metabolism* Epithelial Cells / physiology* Fluorescent Dyes / analysis HeLa Cells Humans Intravital Microscopy* Microscopy, Fluorescence Mitochondrial Proteins / metabolism* Nucleoproteins / metabolism* Staining and Labeling Transcription Factors / metabolism* |
| IF | 3.998 |
| 引用数 | 10 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 21 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HeLa(RCB0007) HeLa/Fucci2(RCB2867) |