RRC ID 49471
著者 Tang Y, Teng JLL, Cheung CLW, Ngan AHY, Huang Y, Wong SSY, Yip EKT, Ng KHL, Que TL, Lau SKP, Woo PCY.
タイトル Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra).
ジャーナル Int J Syst Evol Microbiol
Abstract Two bacterial strains, HKU54T and HKU55, were isolated from the oral cavity of two Chinese cobras (Naja atra) in Hong Kong. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU54T and HKU55, and the two strains shared 99.0 % sequence identities with Tsukamurella inchonensis ATCC 700082T. The two strains had unique biochemical profiles distinguishable from closely related species of the genus Tsukamurella. DNA-DNA hybridization confirmed that they belonged to the same species (≥92.1±7.9 % DNA-DNA relatedness) but were distinct from all other known species of the genus Tsukamurella (≤52.6±5.3 % DNA-DNA relatedness). Chemotaxonomic and morphological analyses of the two strains also demonstrated results consistent with their classification in the genus Tsukamurella. The DNA G+C contents of strains HKU54T and HKU55 were 69.2±1.5 mol% and 69.2±1.3 mol% (mean±sd; n=3) respectively. A novel species, Tsukamurella serpentis sp. nov., is proposed to accommodate strains HKU54T and HKU55, with HKU54T (=JCM 31017T=DSM 100915T) designated as the type strain.
巻・号 66(9)
ページ 3329-3336
公開日 2016-9-1
DOI 10.1099/ijsem.0.001187
PMID 27257031
MeSH Actinomycetales / classification* Actinomycetales / genetics Actinomycetales / isolation & purification Animals Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Elapidae / microbiology* Fatty Acids / chemistry Hong Kong Mouth / microbiology* Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 2.415
引用数 6
リソース情報
一般微生物 JCM 31017 JCM 31018 JCM 30715 JCM 30714 JCM 17688 JCM 15929 JCM 13375 JCM 12608 JCM 15482