| RRC ID |
49498
|
| 著者 |
Yoshida W, Tomikawa J, Inaki M, Kimura H, Onodera M, Hata K, Nakabayashi K.
|
| タイトル |
An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species.
|
| ジャーナル |
PLoS One
|
| Abstract |
Insulators are cis-elements that control the direction of enhancer and silencer activities (enhancer-blocking) and protect genes from silencing by heterochromatinization (barrier activity). Understanding insulators is critical to elucidate gene regulatory mechanisms at chromosomal domain levels. Here, we focused on a genomic region upstream of the mouse Ccnb1ip1 (cyclin B1 interacting protein 1) gene that was methylated in E9.5 embryos of the C57BL/6 strain, but unmethylated in those of the 129X1/SvJ and JF1/Ms strains. We hypothesized the existence of an insulator-type element that prevents the spread of DNA methylation within the 1.8 kbp segment, and actually identified a 242-bp and a 185-bp fragments that were located adjacent to each other and showed insulator and enhancer activities, respectively, in reporter assays. We designated these genomic regions as the Ccnb1ip1 insulator and the Ccnb1ip1 enhancer. The Ccnb1ip1 insulator showed enhancer-blocking activity in the luciferase assays and barrier activity in the colony formation assays. Further examination of the Ccnb1ip1 locus in other mammalian species revealed that the insulator and enhancer are highly conserved among a wide variety of species, and are located immediately upstream of the transcriptional start site of Ccnb1ip1. These newly identified cis-elements may be involved in transcriptional regulation of Ccnb1ip1, which is important in meiotic crossing-over and G2/M transition of the mitotic cell cycle.
|
| 巻・号 |
10(6)
|
| ページ |
e0131204
|
| 公開日 |
2015-1-1
|
| DOI |
10.1371/journal.pone.0131204
|
| PII |
PONE-D-15-03836
|
| PMID |
26110280
|
| PMC |
PMC4481373
|
| MeSH |
Adaptor Proteins, Signal Transducing / genetics*
Adaptor Proteins, Signal Transducing / metabolism
Animals
Cell Cycle Proteins / genetics*
Cell Cycle Proteins / metabolism
Cells, Cultured
DNA Methylation*
Gene Expression Regulation
Genetic Loci
Insulator Elements*
Mice
Mice, Inbred C57BL
Ubiquitin-Protein Ligases
|
| IF |
2.74
|
| 引用数 |
3
|
| リソース情報 |
| 遺伝子材料 |
pGL4-JF1_1-1799 (RDB15327)
pGL4-JF1_1-1799anti (RDB15328)
pGL4-JF1_886-1349 (RDB15329)
pGL4-JF1_886-1349anti (RDB15330)
pGL4-JF1_1128-1312 (RDB15331)
pGL4-JF1_1128-1312anti (RDB15332)
pGL4-JF1_886-1146 (RDB15333)
pGL4-JF1_886-1146anti (RDB15334). |
