RRC ID 49511
Author Hanai A, Ohgi M, Yagi C, Ueda T, Shin HW, Nakayama K.
Title Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis.
Journal J Biochem
Abstract Small GTPases play important roles in various aspects of cell division as well as membrane trafficking. We and others previously showed that ADP-ribosylation factor 6 (Arf6) is locally activated around the ingressing cleavage furrow and recruited to the Flemming body in late cytokinesis phases, and involved in faithful completion of cytokinesis. However, knockout of the Arf6 gene or Arf6 depletion by siRNAs did not drastically influence cytokinesis. We here show that, in addition to Arf6, Class I Arfs (Arf1 and Arf3) are localized to the Flemming body, and that double knockdown of Arf1 and Arf3 moderately increases the proportion of multinucleate cells and simultaneous knockdown of Arf1, Arf3 and Arf6 leads to severe cytokinesis defects. These observations indicate that Arf1 and Arf3 as well as Arf6 play important roles in cytokinesis. We further show that EFA6 (exchange factor for Arf6) activates not only Arf6 but also Arf1 in the cell. Taken together with our previous data, these Arf GTPases are likely to be locally activated by EFA6 and in turn targeted to the Flemming body to complete cytokinesis.
Volume 159(2)
Pages 201-8
Published 2016-2-1
DOI 10.1093/jb/mvv088
PII mvv088
PMID 26330566
PMC PMC4892775
MeSH ADP-Ribosylation Factor 1 / genetics ADP-Ribosylation Factor 1 / metabolism* ADP-Ribosylation Factor 6 ADP-Ribosylation Factors / genetics ADP-Ribosylation Factors / metabolism* Cytokinesis* Gene Knockdown Techniques Gene Knockout Techniques Golgi Apparatus Guanine Nucleotide Exchange Factors / metabolism HeLa Cells Humans Nerve Tissue Proteins / metabolism* Protein Isoforms / genetics Protein Isoforms / metabolism RNA Interference RNA, Small Interfering / genetics
IF 2.476
Times Cited 8
DNA material pcDNA3/hArf5(WT)-HA (RDB15359) pcDNA3/hArf5(Q71L)-HA (RDB15360) pcDNA3/hArf5(T31N)-HA (RDB15361).