RRC ID 49520
Author Sakaue-Sawano A, Kurokawa H, Morimura T, Hanyu A, Hama H, Osawa H, Kashiwagi S, Fukami K, Miyata T, Miyoshi H, Imamura T, Ogawa M, Masai H, Miyawaki A.
Title Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Journal Cell
Abstract The cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We were able to generate cultured cells and transgenic mice constitutively expressing the cell-cycle probes, in which every cell nucleus exhibits either red or green fluorescence. We performed time-lapse imaging to explore the spatiotemporal patterns of cell-cycle dynamics during the epithelial-mesenchymal transition of cultured cells, the migration and differentiation of neural progenitors in brain slices, and the development of tumors across blood vessels in live mice. These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events.
Volume 132(3)
Pages 487-98
Published 2008-2-8
DOI 10.1016/j.cell.2007.12.033
PII S0092-8674(08)00054-8
PMID 18267078
MeSH Animals COS Cells Cell Cycle* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Line Cells, Cultured Cercopithecus aethiops Cytological Techniques* Fluorescence Geminin HeLa Cells Humans Luminescent Proteins / genetics Luminescent Proteins / metabolism Mice Mice, Transgenic Microscopy, Confocal Molecular Sequence Data Morphogenesis Neoplasms / pathology Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Ubiquitination
IF 31.398
Resource
DNA material mKO2-hCdt1(30/120) / pCSII-EF-MCS (RDB15267) mAG-hGeminin(1/110) / pCSII-EF (RDB15268).